Goat F(ab')2 Anti-mouse IgM-Fluorescein Antibody

Catalog #: F0118
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
F0118

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

1 Image

View All Products for IgM

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Goat F(ab')2 Anti-mouse IgM-Fluorescein Antibody Summary

Species Reactivity

Mouse

Specificity

Mouse IgM specific IgG was purified by goat IgG affinity chromatography and then adsorbed to eliminate human cross reactivity. The IgG fraction was digested with pepsin to generate F(ab')2 fragments which have a reduced ability to interact with Fc receptors expressed on a variety of cells.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse IgM heavy and light chains

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter molecule, is of mouse origin and is of the IgM class.The recommended concentration is 10 µL/10⁶ cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgM

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin M

Alternate Names

AGM1; DKFZp686I15196; DKFZp686I15212; FLJ00385; IgM; immunoglobulin heavy constant mu; MGC104996; MGC52291; MU; VH

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

B Cell Markers

Citations for Goat F(ab')2 Anti-mouse IgM-Fluorescein Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

Alterations of bovine nucleus pulposus cells with aging
Authors: Molinos, M;Fiordalisi, MF;Caldeira, J;Almeida, CR;Barbosa, MA;Gonçalves, RM;
Aging cell
Species: Bovine
Sample Types: Whole Cells
Applications: Flow Cytometry
Characterization of mesenchymal progenitor cells isolated from human bone marrow by negative selection.
Authors: Modder UI, Roforth MM, Nicks KM, Peterson JM, McCready LK, Monroe DG, Khosla S
Bone, 2011-12-28;50(3):804-10.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry