Mouse IgM specific IgG was purified by goat IgG affinity chromatography and then adsorbed to eliminate human cross reactivity. The IgG fraction was digested with pepsin to generate F(ab')2 fragments which have a reduced ability to interact with Fc receptors expressed on a variety of cells.
Polyclonal Goat IgG
Supplied in a saline solution containing BSA and Sodium Azide.
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of the IgM class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of MouseIgM Primary Antibody by Flow Cytometry BG01V human embryonic stem cells were stained with MouseAnti-Human TRA-1-81 Monoclonal Antibody (Catalog # MAB8495,filled histogram) or mouse IgM isotype control antibody (open histogram),followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog #F0117) at 10 μL/106 cells.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
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