GOLGB1/Giantin Antibody (PSH03-86)

Western Blot: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381]

Western Blot: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Western blot analysis of GOLGB1/Giantin on different lysates with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/1,000 dilution.

Lane 1: A549 cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: Jurkat cell lysate (20 ug/Lane)
Lane 4: HepG2 cell lysate (20 ug/Lane)
Lane 5: Vero cell lysate (20 ug/Lane)
Lane 6: NIH/3T3 cell lysate (20 ug/Lane)
Lane 7: PC-12 cell lysate (20 ug/Lane)
Lane 8: Mouse lung tissue lysate (40 ug/Lane)
Lane 9: Mouse small intestine tissue lysate (40 ug/Lane)
Lane 10: Rat lung tissue lysate (40 ug/Lane)

Predicted band size: 376 kDa
Observed band size: 376 kDa

Exposure time: Lane 1-6: 5 seconds; Lane 7-10: 1 minute 2 seconds;

3-8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32381) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381]

Immunohistochemistry: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32381) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381]

Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Immunocytochemistry analysis of HeLa cells labeling GOLGB1/Giantin with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.