GOT2 Antibody (3E9)

Novus Biologicals | Catalog # NBP1-47469

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey, Primate

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3E9
View all GOT2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human GOT2 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GOT2 Antibody (3E9)

Western Blot: GOT2 Antibody (3E9)BSA Free [NBP1-47469]

Western Blot: GOT2 Antibody (3E9)BSA Free [NBP1-47469]

Western Blot: GOT2 Antibody (3E9) [NBP1-47469] - Analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: GOT2 Antibody (3E9) - BSA Free [NBP1-47469]

Immunocytochemistry/ Immunofluorescence: GOT2 Antibody (3E9) - BSA Free [NBP1-47469]

Immunocytochemistry/Immunofluorescence: GOT2 Antibody (3E9) [NBP1-47469] - Analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Applications for GOT2 Antibody (3E9)

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GOT2

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Alternate Names

aspartate aminotransferase, mitochondrial, EC 2.6.1, EC 2.6.1.1, FABP-1, FABPpm, Fatty acid-binding protein, FLJ40994, Glutamate oxaloacetate transaminase 2, glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2), KAT4, KATIV, kynurenine aminotransferase IV, mAspAT, mitAAT, Plasma membrane-associated fatty acid-binding protein, Transaminase A

Entrez Gene IDs

2806 (Human); 14719 (Mouse); 25721 (Rat)

Gene Symbol

GOT2

UniProt

P00505 (Human)

Additional GOT2 Products

Product Documents for GOT2 Antibody (3E9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GOT2 Antibody (3E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GOT2 Antibody (3E9)

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Protocols

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FAQs

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