GOT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81565

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GOT2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GFASGDGDKDAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVESQLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKGMADRIIGMRTQLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVER

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GOT2 Antibody - BSA Free

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565] - Analysis in human liver and bone marrow tissues. Corresponding GOT2 RNA-seq data are presented for the same tissues.
Western Blot: GOT2 Antibody [NBP1-81565]

Western Blot: GOT2 Antibody [NBP1-81565]

Western Blot: GOT2 Antibody [NBP1-81565] - Analysis in human cell lines PC-3 and SK-MEL-30. Corresponding GOT2 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565] - Staining of human liver shows very strong cytoplasmic granular positivity in hepatocytes.
Western Blot: GOT2 Antibody [NBP1-81565]

Western Blot: GOT2 Antibody [NBP1-81565]

Western Blot: GOT2 Antibody [NBP1-81565] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565] - Staining of human bone marrow shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565] - Staining of human cerebellum shows strong cytoplasmic granular positivity in cells in granular layer.
Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565] - Staining of human testis shows strong cytoplasmic granular positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP1-81565] - Staining of human small intestine shows moderate cytoplasmic granular positivity in glandular cells.

Applications for GOT2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GOT2

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Alternate Names

aspartate aminotransferase, mitochondrial, EC 2.6.1, EC 2.6.1.1, FABP-1, FABPpm, Fatty acid-binding protein, FLJ40994, Glutamate oxaloacetate transaminase 2, glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2), KAT4, KATIV, kynurenine aminotransferase IV, mAspAT, mitAAT, Plasma membrane-associated fatty acid-binding protein, Transaminase A

Gene Symbol

GOT2

Additional GOT2 Products

Product Documents for GOT2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GOT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GOT2 Antibody - BSA Free

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Protocols

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