GPD2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38542

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GPD2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TAVDMISRRTRLAFLNVQAAEEALPRIVELMGRELNWDDYKKQEQLETARKFLYYEMGYKSRSEQLTDRSEISLLPSDIDRYKKRFHKFDADQKGFITIVDVQRVLESINVQMDENTL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GPD2 Antibody - BSA Free

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining in human parathyroid gland and liver tissues using anti-GPD2 antibody. Corresponding GPD2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human colon, liver, parathyroid gland and skin using Anti-GPD2 antibody NBP2-38542 (A) shows similar protein distribution across tissues to independent antibody NBP1-86121 (B).
Western Blot: GPD2 Antibody [NBP2-38542]

Western Blot: GPD2 Antibody [NBP2-38542]

Western Blot: GPD2 Antibody [NBP2-38542] - Analysis using Anti-GPD2 antibody NBP2-38542 (A) shows similar pattern to independent antibody NBP1-86121 (B).
Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human parathyroid gland shows high expression.
Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human colon.
Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human skin.
Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human liver using Anti-GPD2 antibody NBP2-38542.
Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542]

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human parathyroid gland using Anti-GPD2 antibody NBP2-38542.

Applications for GPD2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPD2

Mitochondrial glycerophosphate dehydrogenase (EC 1.1.99.5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.[supplied by OMIM]

Long Name

Glycerol-3-phosphate dehydrogenase, mitochondrial

Alternate Names

EC 1.1.5.3, GPD-M, GPDH-M, mGDH, mtGPD

Gene Symbol

GPD2

UniProt

P43304 (Human)

Additional GPD2 Products

Product Documents for GPD2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GPD2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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