GPRC5A is a member of the G protein-coupled receptor family, characterized by a signature 7- transmembrane domain motif. GPRC5A is a lung-specific tumor suppressor and may play a role in the interaction between retinoid acid and G protein signaling pathways. GPRC5A is also involved in embryonic development and epithelial cell differentiation.
GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25620
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation, Independent Antibodies, Tagged Protein Expression
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1864 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment of human GPRC5A/RAI3.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free
Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] -
Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] -
Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. RAI3 stained by RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] -
Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) [NBP3-25620] -
GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.
GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence: GPRC5A/RAI3 Antibody (HL1864) [NBP3-25620] -
GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunofluorescent analysis.Sample: Wild-type (WT) and GPRC5A/RAI3 knockout (KO) MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:500.
Blue: Fluoroshield with DAPI
Western Blot: GPRC5A/RAI3 Antibody (HL1864) [NBP3-25620] -
Boiled and unboiled wild-type (WT) and GPRC5A/RAI3 knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.
Western Blot: GPRC5A/RAI3 Antibody (HL1864) [NBP3-25620] -
Non-transfected (-) and transfected (+) unboiled MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.
Western Blot: GPRC5A/RAI3 Antibody (HL1864) [NBP3-25620] -
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunocytochemistry/ Immunofluorescence: GPRC5A/RAI3 Antibody (HL1864) [NBP3-25620] -
GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein at cell membrane by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) [NBP3-25620] -
GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.
GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100 and highly cited competitor's antibody diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min*Competitor's antibody is not affiliated withand does not endorse this product.
Western Blot: GPRC5A/RAI3 Antibody (HL1864) [NBP3-25620] -
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) [NBP3-25620] -
GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.
GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: GPRC5A/RAI3 Antibody (HL1864) [NBP3-25620] -
Unboiled non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GPRC5A
Long Name
G Protein-coupled Receptor, Family C, Group 5, Member A
Alternate Names
RAI3, RAIG1
Gene Symbol
GPRC5A
Additional GPRC5A Products
Product Documents for GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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