Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.
Granzyme D Antibody (RM0246-8G14) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-12409
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2 Clone # RM0246-8G14
Format
Azide and BSA Free
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Product Specifications
Immunogen
Mouse recombinant Granzyme D
Clonality
Monoclonal
Host
Rat
Isotype
IgG2
Applications for Granzyme D Antibody (RM0246-8G14) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:50-1:200
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Reconstitution
Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: Granzyme D
Additional Granzyme D Products
Product Documents for Granzyme D Antibody (RM0246-8G14) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Granzyme D Antibody (RM0246-8G14) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Granzyme D Antibody (RM0246-8G14) - Azide and BSA Free
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Q: I would like to enquire about the specificity of the rat monoclonal antibody against mouse granzyme D (RM0246-8G14). Do you have any information on its cross reactivity with other members of the granzyme family?
A: This antibody hasn't specifically been tested for cross reactivity to other members of the granzyme family. It was generated against full-length Granzyme D. Granzyme D shares 89% homology to Granzyme E, 77% similarity to Granzyme G and 73% similarity to Granzyme F.
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