GRASP65 Antibody (OTI5G8)

Novus Biologicals | Catalog # NBP2-02665

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Porcine, Canine, Monkey, Primate

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5G8
View all GRASP65 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GORASP1 (NP_114105) produced in HEK293T cell.

Reactivity Notes

Porcine reactivity reported from a verified customer review.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRASP65 Antibody (OTI5G8)

Western Blot: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Western Blot: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Western Blot: GRASP65 Antibody (5G8) [NBP2-02665] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GRASP65 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665] - Staining in Hela cells: red CFTR, Green GRASP65 and Blue is DAPI. Image from a verified customer review.
Immunohistochemistry-Paraffin: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunohistochemistry-Paraffin: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human prostate tissue using anti-GRASP65 mouse monoclonal antibody.
Flow Cytometry: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Flow Cytometry: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Flow Cytometry: GRASP65 Antibody (5G8) [NBP2-02665] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRASP65 antibody, and then analyzed by flow cytometry.
Western Blot: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Western Blot: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Western Blot: GRASP65 Antibody (5G8) [NBP2-02665] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRASP65 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRASP65.
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRASP65.
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665] - GRASP65 Antibody (5G8) [NBP2-02665] - Golgi localization in human Wi26 cells. Works for ICC on MeOH and PFA-fixed cells 1:100-1:200 dilution WB on human lung fibroblasts 1:2000 in TBST (membrane blocking with 5% skimmed milk). This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665] - Detection in cultured pig trabecular meshwork cells. Image from a verified customer review.
Immunohistochemistry-Paraffin: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunohistochemistry-Paraffin: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GRASP65 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunohistochemistry-Paraffin: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human Kidney tissue using anti-GRASP65 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunohistochemistry-Paraffin: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human pancreas tissue using anti-GRASP65 mouse monoclonal antibody.
Flow Cytometry: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Flow Cytometry: GRASP65 Antibody (OTI5G8) [NBP2-02665]

Flow Cytometry: GRASP65 Antibody (5G8) [NBP2-02665] - Analysis of Jurkat cells, using anti-GRASP65 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for GRASP65 Antibody (OTI5G8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000-2000

Reviewed Applications

Read 3 reviews rated 4.7 using NBP2-02665 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.6 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRASP65

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. [provided by RefSeq].

Alternate Names

Golgi peripheral membrane protein p65, Golgi phosphoprotein 5MGC118897, golgi reassembly stacking protein 1, 65kDa, Golgi reassembly-stacking protein 1, Golgi reassembly-stacking protein of 65 kDa, GRASP65MGC118894, P6565 kDa

Entrez Gene IDs

64689 (Human)

Gene Symbol

GORASP1

Additional GRASP65 Products

Product Documents for GRASP65 Antibody (OTI5G8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GRASP65 Antibody (OTI5G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GRASP65 Antibody (OTI5G8)

Customer Reviews for GRASP65 Antibody (OTI5G8) (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • GRASP65 Antibody (5G8)
    Name: Julian Nuechel
    Application: Immunocytochemistry
    Sample Tested: Human fibroblast cell line
    Species: Human
    Verified Customer | Posted 07/11/2017
    Golgi localization in human Wi26 cells
    Works for IF on MeOH and PFA-fixed cells 1:100-1:200 dilution WB on human lung fibroblasts 1:2000 in TBST (membrane blocking with 5% skimmed milk)
    GRASP65 Antibody (OTI5G8) NBP2-02665
  • GRASP65 Antibody (5G8)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: abecular meshwork cells
    Species: Pig
    Verified Customer | Posted 11/28/2016
    Detection of GRASP65 in cultured pig trabecular meshwork cells using GRASP65 Antibody (5G8) (NBP2-02665) at 1:500 dilution overnight and donkey anti-mouse Alexa 594 (1:1000) for 1 hour.
    GRASP65 Antibody (OTI5G8) NBP2-02665
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 09/22/2015
    Hela Cells
    GRASP65 Antibody (OTI5G8) NBP2-02665

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Protocols

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