GRK1 Antibody (D11) [Alexa Fluor™ Plus 647]

Novus Biologicals | Catalog # NB120-2776AFP647

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Bovine

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Alexa Fluor Plus 647

Antibody Source

Monoclonal Mouse IgG1 Clone # D11
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Product Specifications

Immunogen

Full length human GRK1.

Epitope

This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.

Specificity

Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This does not cross-react with other G protein-coupled kinases.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for GRK1 Antibody (D11) [Alexa Fluor™ Plus 647]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GRK1

The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.

Long Name

G protein-coupled Receptor Kinase 1

Alternate Names

GPRK1, RHOK

Gene Symbol

GRK1

Additional GRK1 Products

Product Documents for GRK1 Antibody (D11) [Alexa Fluor™ Plus 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GRK1 Antibody (D11) [Alexa Fluor™ Plus 647]



This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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