Amino Acid Sequence: MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

21.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli GrpE Protein

SDS-PAGE: Recombinant E. coli GrpE Protein [NBC1-18370]

SDS-Page: Recombinant E. coli GrpE Protein [NBC1-18370] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBC1-18370

Formulation	20 mM Tris-HCl buffer (pH 8.0), 100 mM NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GrpE

GrpE,co-chaperone of E.coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E.coli and purified by using the conventional column chromatography techniques.

Alternate Names

Heat shock protein B25.3, HSP24, HSP-70 cofactor

Gene Symbol

grpE

Additional GrpE Products

Product Documents for Recombinant E. coli GrpE Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant E. coli GrpE Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant E. coli GrpE Protein

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Recombinant E. coli GrpE Protein

Novus Biologicals | Catalog # NBC1-18370

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Description