GST Epitope Tag Antibody (3G10) - Azide and BSA Free

Novus Biologicals | Catalog # NB600-446

Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3G10

Format

Azide and BSA Free
View all GST Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

GST protein of the parasitic helminth Schistosoma japonicum expressed in E. coli

Specificity

Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GST Epitope Tag Antibody (3G10) - Azide and BSA Free

Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446]

Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446]

Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446] - A. 2 ug His-recombinant protein B. 2 ug GST-recombinant protein C. 1 ug GST-recombinant protein D. 0.5 ug GST-recombinant protein 10 % SDS-PAGE GST tag antibody [3G10] dilution: 1:5000
Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446]

Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446]

Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446] - A. Non-induced cell lysate from E. coli expressing GST-tagged proteinsB. IPTG-induced cell lysate from E. coli expressing GST-tagged proteins10 % SDS-PAGE GST tag antibody [3G10] dilution: 1:1000

Applications for GST Epitope Tag Antibody (3G10) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10 - 1:500

Western Blot

0.1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GST Epitope Tag

Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.

Alternate Names

EC 2.5.1.18, glutathione S-alkyltransferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase M1, glutathione S-transferase mu 1, GST class-mu 1, GST HB subunit 4, GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, HB subunit 4, MGC26563, MU, MU-1, S-(hydroxyalkyl)glutathione lyase

Gene Symbol

GSTM1

UniProt

P09488

Additional GST Epitope Tag Products

Product Documents for GST Epitope Tag Antibody (3G10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GST Epitope Tag Antibody (3G10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GST Epitope Tag Antibody (3G10) - Azide and BSA Free

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