GST Epitope Tag Antibody (8-326)

Novus Biologicals | Catalog # NBP2-37824

Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8-326
View all GST Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

Recombinant purified full-length GST

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GST Epitope Tag Antibody (8-326)

Western Blot: GST Epitope Tag Antibody (8-326) [NBP2-37824]

Western Blot: GST Epitope Tag Antibody (8-326) [NBP2-37824]

Western Blot: GST Epitope Tag Antibody (8-326) [NBP2-37824] - Analysis of GST-Abl SH2 domain (Lane 1, 35kDa) and GST-Grb2 (Lane 2, 38kDa) SH2 domain was performed by loading 2ug of each purified fusion protein.
Immunoprecipitation: GST Epitope Tag Antibody (8-326) [NBP2-37824]

Immunoprecipitation: GST Epitope Tag Antibody (8-326) [NBP2-37824]

Immunoprecipitation: GST Epitope Tag Antibody (8-326) [NBP2-37824] - Analysis of GST-Shp2 SH2 domain (Lane 2, 39kDa) was performed by mixing 2ug of purified fusion protein with 1 ug of mouse monoclonal antibody recognizing GST overnight on a rocking platform at 4C.

Applications for GST Epitope Tag Antibody (8-326)

Application
Recommended Usage

Immunoprecipitation

2 ug

Simple Western

1:100

Western Blot

1:1000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Human melanoma cells, separated by Size, antibody dilution of 1:100, apparent MW was Various kDa

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GST Epitope Tag

Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.

Alternate Names

EC 2.5.1.18, glutathione S-alkyltransferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase M1, glutathione S-transferase mu 1, GST class-mu 1, GST HB subunit 4, GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, HB subunit 4, MGC26563, MU, MU-1, S-(hydroxyalkyl)glutathione lyase

Gene Symbol

GSTM1

Additional GST Epitope Tag Products

Product Documents for GST Epitope Tag Antibody (8-326)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GST Epitope Tag Antibody (8-326)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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