HB-EGF Antibody (DMC500) - Chimeric

Novus Biologicals | Catalog # NBP3-48376

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # DMC500
View all HB-EGF Primary Antibodies »

Product Specifications

Immunogen

HB-EGF recombinant protein expressed in mammalian cells (Accession# Q99075). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for HB-EGF Antibody (DMC500) - Chimeric

HB-EGF Antibody (DMC500) - Chimeric

Flow Cytometry: HB-EGF Antibody (DMC500) - Chimeric [NBP3-48376]

Flow cytometry analysis of antigen binding of anti-human HBEGF mAb(NBP3-48376). (A) NBP3-48376 does not bind to CHO-S cells that do not express HBEGF. (B) A clear peak shift of NBP3-48376 was seen compared to the control when incubated with HBEGF-expressing MDA-MB-231 cells, indicating strong binding of NBP3-48376 to HBEGF. Antibodies were incubated at 5 ug/mL.
HB-EGF Antibody (DMC500) - Chimeric

Flow Cytometry: HB-EGF Antibody (DMC500) - Chimeric [NBP3-48376]

Flow cytometry analysis with Anti-HB-EGF(DMC500) on HEK293 cells transfected with human HB-EGF (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Applications for HB-EGF Antibody (DMC500) - Chimeric

Application
Recommended Usage

Flow Cytometry

1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HB-EGF

HB-EGF is an EGF related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. HB-EGF is expressed in numerous cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF. Recombinant human HB-EGF is a 9.7 kDa protein containing 86 amino acid residues, corresponding to the extra-cellular EGF-like and heparin binding domains of the full length HB-EGF protein.

Long Name

Heparin Binding EGF-like Growth Factor

Alternate Names

Dtr, Dts, HBEGF, Hegfl

Gene Symbol

HBEGF

Additional HB-EGF Products

Product Documents for HB-EGF Antibody (DMC500) - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HB-EGF Antibody (DMC500) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

EGF Family

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