HBC 530

Catalog # Availability Size / Price Qty
HBC 530 | CAS No. 156840-13-0 | Fluorescent RNA Probes
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Description: GFP fluorophore mimic for imaging RNA in live cells; activated by binding to Pepper aptamers; suitable for confocal and two-photon microscopy

Chemical Name: 4-Cyano-α-[[4-[(2-hydroxyethyl)methylamino]phenyl]methylene]benzeneacetonitrile

Purity: ≥98%

Product Details

Biological Activity

HBC 530 is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in live cells. Fluoresces when bound to Pepper aptamer; displays high affinity binding (Kd = 3.5 nM). Suitable for confocal and two-photon microscopy. Confocal excitation maximum = 488 nm, emission maximum= 495 - 595 nm. Two-photon excitation maximum = 976 nm, emission maximum= 475 - 575 nm.

To make a 1000x stock solution, add 200 μL DMSO (Cat. No. 3176).

Optical Data for HBC 530

λabs 485 nm
λem 530 nm
Extinction Coefficient (ε) 65300 M-1cm-1
Quantum Yield (φ) 0.66
Closest Laser line 488 nm
Application Fluorogen, used with RNA light-up aptamer
Alternative For Cat. No. 6434

Technical Data

Soluble to 100 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.
    Chen et al.
    Nat.Biotechnol., 2019;37:1287

Product Datasheets

Certificate of Analysis is currently unavailable on-line. Please contact Customer Service
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