HBC 530
Tocris Bioscience | Catalog # 7277
Key Product Details
Description
Wavelength
Product Description
HBC 530 is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in live cells. Fluoresces when bound to Pepper aptamer; displays high affinity binding (Kd = 3.5 nM). Suitable for confocal and two-photon microscopy. Confocal excitation maximum = 488 nm, emission maximum = 495 - 595 nm. Two-photon excitation maximum = 976 nm, emission maximum = 475 - 575 nm.
To make a 1000x stock solution, add 200 μL DMSO (Cat. No. 3176).
Optical Data
| λabs | 485 nm |
| λem | 530 nm |
| Extinction Coefficient (ε) | 65300 M-1cm-1 |
| Quantum Yield (φ) | 0.66 |
| Closest Laser line | 488 nm |
| Application | Fluorogen, used with RNA Light-up Aptamer, Confocal Microscopy |
| Alternative For | Cat. No. 6434 |
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Product Specifications for HBC 530
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 30.34 | 100 |
Preparing Stock Solutions for HBC 530
The following data is based on the product molecular weight 303.37.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.30 mL | 16.48 mL | 32.96 mL |
| 5 mM | 0.66 mL | 3.30 mL | 6.59 mL |
| 10 mM | 0.33 mL | 1.65 mL | 3.30 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.66 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Chen Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat.Biotechnol. 2019 PMID: 31548726
Product Documents for HBC 530
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HBC 530
For research use only
Citations for HBC 530
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