Human HBeAg ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60029

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human HBeAg. No significant cross-reactivity or interference between human HBeAg and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 15

Inter-Assay Precision (Precision between assays) %CV 20

Kit Contents for Human HBeAg ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Coated assay plate
  • HRP-conjugate
  • Instruction manual
  • Negative Control
  • Positive Control
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HBeAg

This assay employs the qualitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HBeAb. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated HBeAb. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HBeAg bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

HBeAg

Additional HBeAg Products

Product Documents for Human HBeAg ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human HBeAg ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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