HCLS1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16787

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (95%), Mouse (95%), Rat (95%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HCLS1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HCLS1. The exact sequence is proprietary.

Reactivity Notes

Chicken (82%).

Localization

Membrane; Peripheral membrane protein (Probable); Cytoplasm (Probable); Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HCLS1 Antibody - BSA Free

Western Blot: HCLS1 Antibody [NBP2-16787]

Western Blot: HCLS1 Antibody [NBP2-16787]

Western Blot: HCLS1 Antibody [NBP2-16787] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.
HCLS1 Antibody - BSA Free

Immunoprecipitation: HCLS1 Antibody [NBP2-16787] -

HCLS1 antibody immunoprecipitates HCLS1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of HCLS1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of HCLS1 by 2.5 ug of HCLS1 antibody (NBP2-16787) 10% SDS-PAGE The immunoprecipitated HCLS1 protein was detected by HCLS1 antibody (NBP2-16787) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Applications for HCLS1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HCLS1

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.

Long Name

Hematopoietic Cell-specific Lyn Substrate 1

Alternate Names

Cortactin-like, CTTNL, LckBP1

Gene Symbol

HCLS1

UniProt

P14317

Additional HCLS1 Products

Product Documents for HCLS1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HCLS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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