Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HDAC10 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to a portion of human HDAC 10.

Reactivity Notes

Human.

Specificity

Reacts with the human HDAC 10 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HDAC10 Antibody - BSA Free

Western Blot: HDAC10 AntibodyBSA Free [NB600-223]

Western Blot: HDAC10 AntibodyBSA Free [NB600-223]

Western Blot: HDAC10 Antibody [NB600-223] - WB analysis of HDAC10 in HeLa cells with NB600-223 at a dilution of 1:250.
Simple Western: HDAC10 AntibodyBSA Free [NB600-223]

Simple Western: HDAC10 AntibodyBSA Free [NB600-223]

Simple Western: HDAC10 Antibody [NB600-223] - 1:25 dilution against the indicated concentration of protein lysate (Human SVG-A). Image from verified customer review.

Applications for HDAC10 Antibody - BSA Free

Application
Recommended Usage

Simple Western

1:25

Western Blot

1:500-1:1000
Application Notes

Western blot, a predominant band is seen at ~65-70 kDa; it has been reported that HDAC10 variants seem present in certain cell lines and tissues that are as of yet uncharacterized. Other bands may be seen below 50 kDa, and in some cases they are predominant. Simple Western reported in verified customer review.
See Simple Western Antibody Database for Simple Western validation: Tested in Human SVG-A Protein Lysate, separated by Size, antibody dilution of 1:25

Reviewed Applications

Read 1 review rated 4 using NB600-223 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HDAC10

A novel class II histone deacetylase, HDAC10 is most similar to HDAC6. Both contain a unique, putative second catalytic domain not found in other HDACs. In HDAC10, however, this domain is not functional. Unlike HDAC6, which is normally a cytoplasmic deacetylase, HDAC10 resides in both the nucleus and cytoplasm. Data suggests that HDAC10 may play roles both in the nucleus, as a transcriptional modulator, and in the cytoplasm in an unidentified role.

Long Name

Histone deacetylase 10

Alternate Names

EC 3.5.1.48, EC 3.5.1.62, HD10

Entrez Gene IDs

83933 (Human)

Gene Symbol

HDAC10

Additional HDAC10 Products

Product Documents for HDAC10 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HDAC10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for HDAC10 Antibody - BSA Free (1)

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Customer Images

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  • HDAC10 Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: B103 cells, SVG-A whole cell lysate and B103 whole cell lysate
    Species: Rat and Human
    Verified Customer | Posted 03/08/2019
    Antibody tested by Simple Western at 1:25 dilution against the indicated concentration of protein lysate, in two cell lines (rat B103 or human SVG-A).
    HDAC10 Antibody - BSA Free NB600-223

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