Human Heart Whole Tissue Lysate (Adult Whole Normal)
Novus Biologicals | Catalog # NB820-59217
Key Product Details
Species
Applications
Product Specifications
Application Notes
Specificity
Type
Life Stage
Tissue
Protein State
Tissue Condition
Subcellular Fraction
Formulation, Preparation, and Storage
Formulation
Concentration
Shipping
Storage
Background: Heart
Additional Heart Products
Product Documents for Human Heart Whole Tissue Lysate (Adult Whole Normal)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Human Heart Whole Tissue Lysate (Adult Whole Normal)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
Citations for Human Heart Whole Tissue Lysate (Adult Whole Normal)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Human Heart Whole Tissue Lysate (Adult Whole Normal)
-
Q: I was looking at NB820-59217 and I would like to know if it is denatured at all?
A: They will not be native since they were lysed in this buffer:
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
with detergents -
Q: How much protein do you recommend to use for each WB well for best results with a standard gel of 12 wells?
A: We recommend loading 10 to 20 ug per well.
-
Q: If I want to dilute the 20ug so it will give a volume larger than 4ul as supplied, what buffer do you recommend to use for that?
A: We recommend diluting in the buffer it comes supplied in, HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
-
Q: Is this lysate a whole heart tissue or a particular heart myocard area - ventricle / atrium?
A: NB820-59217 is a lysate from the whole heart.
-
Q: I was looking at NB820-59217 and I would like to know if it is denatured at all?
A: They will not be native since they were lysed in this buffer:
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
with detergents -
Q: How much protein do you recommend to use for each WB well for best results with a standard gel of 12 wells?
A: We recommend loading 10 to 20 ug per well.
-
Q: If I want to dilute the 20ug so it will give a volume larger than 4ul as supplied, what buffer do you recommend to use for that?
A: We recommend diluting in the buffer it comes supplied in, HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
-
Q: Is this lysate a whole heart tissue or a particular heart myocard area - ventricle / atrium?
A: NB820-59217 is a lysate from the whole heart.
-
Q: I was looking at NB820-59217 and I would like to know if it is denatured at all?
A: They will not be native since they were lysed in this buffer:
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
with detergents -
Q: How much protein do you recommend to use for each WB well for best results with a standard gel of 12 wells?
A: We recommend loading 10 to 20 ug per well.
-
Q: If I want to dilute the 20ug so it will give a volume larger than 4ul as supplied, what buffer do you recommend to use for that?
A: We recommend diluting in the buffer it comes supplied in, HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
-
Q: Is this lysate a whole heart tissue or a particular heart myocard area - ventricle / atrium?
A: NB820-59217 is a lysate from the whole heart.
-
Q: I was looking at NB820-59217 and I would like to know if it is denatured at all?
A: They will not be native since they were lysed in this buffer:
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
with detergents -
Q: How much protein do you recommend to use for each WB well for best results with a standard gel of 12 wells?
A: We recommend loading 10 to 20 ug per well.
-
Q: If I want to dilute the 20ug so it will give a volume larger than 4ul as supplied, what buffer do you recommend to use for that?
A: We recommend diluting in the buffer it comes supplied in, HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
-
Q: Is this lysate a whole heart tissue or a particular heart myocard area - ventricle / atrium?
A: NB820-59217 is a lysate from the whole heart.