Recombinant Human Hemoglobin A2 His Protein

Novus Biologicals | Catalog # NBP2-23111

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all Hemoglobin A2 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-142 of Human Hemoglobin A2

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMVL SPADKTNVKA AWGKVGAHAG EYGAEALERM FLSFPTTKTY FPHFDLSHGS AQVKGHGKKV ADALTNAVAH VDDMPNALSA LSDLHAHKLR VDPVNFKLLS HCLLVTLAAH LPAEFTPAVH ASLDKFLASV STVLTSKYR

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Hemoglobin A2 His Protein

SDS-PAGE: Recombinant Human Hemoglobin A2 His Protein [NBP2-23111]

SDS-PAGE: Recombinant Human Hemoglobin A2 His Protein [NBP2-23111]

SDS-Page: Recombinant Human Hemoglobin A2 Protein [NBP2-23111]

Formulation, Preparation, and Storage

NBP2-23111
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 20% glycerol, 2M UREA, 2 mM DTT
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hemoglobin A2

Hemoglobin subunit alpha, also known as HBA2, belongs to the globin family. HBA2 is involved in oxygen transport from the lung to the various peripheral tissues. The alpha-2(HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. Recombinant human HBA2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

alpha globin, alpha-2 globin, alpha-globin, Hemoglobin alpha chain, hemoglobin subunit alpha, hemoglobin, alpha 2

Gene Symbol

HBA2

Additional Hemoglobin A2 Products

Product Documents for Recombinant Human Hemoglobin A2 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human Hemoglobin A2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human Hemoglobin A2 His Protein

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