Hemoglobin is a tetrameric protein that serves as the oxygen transporter in red blood cells. Two alpha chain and two beta chains comprise the adult form of the molecule, with each subunit containing a heme group, or oxygen binding site. Sickle cell anemia is caused by a single amino acid change in the beta chain, which alters the solubility of the deoxygenated form of the molecule and causes the formation of long fibers that distort the red blood cell into the characteristic sickle shape. Thalassemias, another set of genetic diseases associated with hemoglobin, result from absence or deficiency in one of the hemoglobin chains.
Human Hemoglobin ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP3-12175
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Key Product Details
Sample Type & Volume Required Per Well
Whole Blood, Serum, Plasma, Cerebrospinal Fluid (CSF), Cell Culture Media
Assay Range
0.27 - 200 ng/mL
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Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Human
Description
Detection method: Biotinylated-Streptavidin-HRP
Scientific Data Images for Human Hemoglobin ELISA Kit (Colorimetric)
ELISA: Human Hemoglobin ELISA Kit (Colorimetric) [NBP3-12175]
ELISA: Human Hemoglobin ELISA Kit [NBP3-12175] - Typical standard curve.Kit Contents for Human Hemoglobin ELISA Kit (Colorimetric)
- Dilution Buffer B (10X) - 25 mL
- HRP Solution A - 12 mL
- Human Hemoglobin Detection Antibody - 12 mL
- Human Hemoglobin Pre-Coated 96-well Strip Plate - 1 EA
- Human Hemoglobin Standard (200 ng / vial) - 2 EA
- Sealing Tape - 6 sheets
- Stop Solution - 12 mL
- TMB Substrate - 12 mL
- Wash Buffer (20X) - 12 mL
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4°C.
Background: Hemoglobin
Alternate Names
alpha one globin;alpha-1 globin;alpha-1-globin;alpha-2 globin chain;Alpha-globin;ALPHA-GLOBIN GENE;delta globin;Haemoglobin;Hb;HbA1c;hemoglobin alpha 1 globin chain;Hemoglobin alpha chain;Hemoglobin subunit alpha;hemoglobin, alpha 1
Additional Hemoglobin Products
Product Documents for Human Hemoglobin ELISA Kit (Colorimetric)
Certificate of Analysis
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Product Specific Notices for Human Hemoglobin ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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