Hepatocyte Specific Antigen Antibody (HSA133)

Novus Biologicals | Catalog # NBP2-32861

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # HSA133
View all Hepatocyte Specific Antigen Primary Antibodies »

Product Specifications

Immunogen

SK-H1A9-2 human hepatocellular carcinoma cells

Localization

Cell surface

Marker

Hepatocellular Marker

Specificity

monoclonal antibody HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this monoclonal antibody on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34644)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for Hepatocyte Specific Antigen Antibody (HSA133)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
ELISA: For coating, purchase antibody without BSA.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Hepatocyte Specific Antigen

HSA (Human Serum Albumin) is useful in studying hepatocellular tumors. It may be useful in differentiating clear cell hepatocellular carcinomas from other clear cell malignancies. It has been shown in the literature to be useful in differentiating hepatoblastoma of embryonal type from small round cell tumors of childhood. It labels an antigen in the mitochondrial fraction of the liver homogenates.

Alternate Names

Hepatocyte, HSA

Additional Hepatocyte Specific Antigen Products

Product Documents for Hepatocyte Specific Antigen Antibody (HSA133)

Certificate of Analysis

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Product Specific Notices for Hepatocyte Specific Antigen Antibody (HSA133)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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