Herpes Simplex Virus VP16 Antibody [mFluor Violet 450 SE]

Novus Biologicals | Catalog # NB600-950MFV450

Novus Biologicals
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Key Product Details

Species Reactivity

Virus

Applications

Western Blot, Immunoprecipitation

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Polyclonal Rabbit IgG
View all Herpes Simplex Virus VP16 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus Herpes Simplex Virus VP16 protein, conjugated to KLH via an N-terminal added cysteine.

Reactivity Notes

Tag specific

Specificity

Recognizes Herpes Simplex Virus VP16 fusion protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Herpes Simplex Virus VP16 Antibody [mFluor Violet 450 SE]

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Herpes Simplex Virus VP16

VP16 from herpes simple virus (HSV) is a strong transcriptional activator of immediate early viral genes.1 Due to its lack of DNA binding activity, VP16 is recruited to DNA via interaction with DNA binding proteins.2 Ptashne et al. showed that VP16 can function as a potent transcriptional activator in mammalian cells when fused to the DNA binding domain of the yeast transcriptional activator GAL4.3 These properties were taken into consideration in the development of a mammalian version of the Yeast Two-Hybrid System.4-6 The Yeast Two-Hybrid System is a powerful method for characterizing and screening for protein-protein interactions. In the original version of the system, GAL4 DNA binding domain is fused to protein X, and GAL4 activation domain is fused to protein Y. Neither hybrid is capable of activating transcription individually. Upon interaction between proteins X and Y, the DNA binding and activator domains are brought in close proximity. As a result, the transcriptional activity of GAL4 is reconstituted at the promoter, and monitored with a reporter gene.4 Additional two-hybrid systems were developed using different DNA binding and activation domains as well as different reporter genes.5-9 The VP16 activator is used in yeast and mammalian systems. In yeast, its DNA interaction partner is typically LexA.10 In mammalian systems, interaction between two proteins is detected through recruitment of VP16 and GAL4 DNA binding domain to GAL4 operators present in the reporter gene.7,9-11

Additional Herpes Simplex Virus VP16 Products

Product Documents for Herpes Simplex Virus VP16 Antibody [mFluor Violet 450 SE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Herpes Simplex Virus VP16 Antibody [mFluor Violet 450 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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