Hexokinase 2 Antibody (HL2000)

Novus Biologicals | Catalog # NBP3-48667

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2000
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Product Specifications

Immunogen

Recombinant fragment of human Hexokinase 2 II.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Hexokinase 2 Antibody (HL2000)

Hexokinase 2 Antibody (HL2000)

Western Blot: Hexokinase 2 Antibody (HL2000) [NBP3-48667] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Hexokinase 2 Antibody (HL2000)

Western Blot: Hexokinase 2 Antibody (HL2000) [NBP3-48667] -

Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Hexokinase 2 Antibody (HL2000)

Western Blot: Hexokinase 2 Antibody (HL2000) [NBP3-48667] -

Zebrafish tissue extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Hexokinase 2 Antibody (HL2000)

Western Blot: Hexokinase 2 Antibody (HL2000) [NBP3-48667] -

Whole Japanese medaka extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Hexokinase 2 antibody [HL2000] (NBP3-48667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for Hexokinase 2 Antibody (HL2000)

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hexokinase 2

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Alternate Names

HK2, HKII, HXK2

Gene Symbol

HK2

Additional Hexokinase 2 Products

Product Documents for Hexokinase 2 Antibody (HL2000)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Hexokinase 2 Antibody (HL2000)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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