Hexosaminidase A/HEXA Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-74127

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Hexosaminidase A/HEXA Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to the C terminal of HEXA. Immunizing peptide sequence: WKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAV. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27466344).

Specificity

This product is specific to Subunit or Isoform: alpha.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Hexosaminidase A/HEXA Antibody - BSA Free

Western Blot: Hexosaminidase A/HEXA Antibody [NBP1-74127]

Western Blot: Hexosaminidase A/HEXA Antibody [NBP1-74127]

Western Blot: Hexosaminidase A/HEXA Antibody [NBP1-74127] - Titration: 1 ug/ml Positive Control: Fetal kidney lysate.
Immunohistochemistry-Paraffin: Hexosaminidase A/HEXA Antibody [NBP1-74127]

Immunohistochemistry-Paraffin: Hexosaminidase A/HEXA Antibody [NBP1-74127]

Immunohistochemistry-Paraffin: Hexosaminidase A/HEXA Antibody [NBP1-74127] - Human Brain, cortex tissue at an antibody concentration of 5ug/ml.

Applications for Hexosaminidase A/HEXA Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

2-5 ug/ml

Western Blot

1.0 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID 27466344).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hexosaminidase A/HEXA

This gene encodes the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Alpha subunit gene mutations lead to Tay-Sachs disease (GM2-gangliosidosis type I).

Alternate Names

HEXA, TSD

Gene Symbol

HEXA

UniProt

P06865

Additional Hexosaminidase A/HEXA Products

Product Documents for Hexosaminidase A/HEXA Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Hexosaminidase A/HEXA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Hexosaminidase A/HEXA Antibody - BSA Free

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Protocols

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