HGS Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83202

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HGS Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LAEDIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHAAPTNVVENPLPETDSQPIPPSGGPFSEPQFHNGESEESHEQFLKALQNAVTTFVNRMKSNHMRGRSITNDS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HGS Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HGS Antibody [NBP1-83202]

Immunocytochemistry/ Immunofluorescence: HGS Antibody [NBP1-83202]

Immunocytochemistry/Immunofluorescence: HGS Antibody [NBP1-83202] - Staining of human cell line U-2 OS shows localization to cytosol & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83202]

Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83202]

Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83202] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83202]

Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83202]

Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83202] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83202]

Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83202]

Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83202] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83202]

Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83202]

Immunohistochemistry-Paraffin: HGS Antibody [NBP1-83202] - Staining of human cerebral cortex shows moderate positivity in neuropil.
HGS Antibody - BSA Free Western Blot: HGS Antibody - BSA Free [NBP1-83202]

Western Blot: HGS Antibody - BSA Free [NBP1-83202]

Analysis in control (vector only transfected HEK293T lysate) and HGS over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for HGS Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HGS

HGS is involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early end

Alternate Names

FLJ39878, hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, HRSFLJ11421, human growth factor-regulated tyrosine kinase substrate, Protein pp110, Vps27, ZFYVE8

Gene Symbol

HGS

Additional HGS Products

Product Documents for HGS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HGS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HGS Antibody - BSA Free

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Protocols

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FAQs

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