High Throughput Glutathione Peroxidase Assay Kit

High Throughput Glutathione Peroxidase Assay Kit Summary

Key Benefits

• Suitable for a 96 well or cuvette format
• Suitable for plasma, erythrocyte lysates, tissue homogenates, and cell lysates

Why Use the HT Glutathione Peroxidase Assay Kit?

Tetrameric Glutathione Peroxidase catalyzes the reduction of H2O2 to water, and organic peroxides to the corresponding stable alcohols, by using glutathione as a source of reducing equivalents. It requires selenium as a cofactor and contains a selenocysteine amino acid residue in the active site of each monomer that participates in the actual mechanism of the enzyme. Glutathione Reductase (GR), provided with each kit, then reduces the oxidized glutathione to complete the cycle. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the absorbance at 340 nm is directly proportional to the Glutathione Peroxidase activity in the sample.

Other Resources

7512-100-k_cuvette_assay_calculation_worksheet7512-100-k_plate_assay_calculation_worksheet

Kit Contents

• Glutathione Peroxidase
• 10X Assay Buffer
• GSH+NADPH
• Glutathione Reductase
• Cumene Hydroperoxide
• 96-well plates

Specifications

Limitations

For research use only. Not for diagnositic use.

Product Datasheets