High Throughput Glutathione Peroxidase Assay Kit
R&D Systems | Catalog # 7512-100-K
Quantification of glutathione peroxidase activity.
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Key Product Details
Features
Measures glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.
Key Benefits
- Suitable for a 96 well or cuvette format
- Suitable for plasma, erythrocyte lysates, tissue homogenates, and cell lysates
Species
Multi-Species
Product Summary for High Throughput Glutathione Peroxidase Assay Kit
Why Use the HT Glutathione Peroxidase Assay Kit?
Tetrameric Glutathione Peroxidase catalyzes the reduction of H2O2 to water, and organic peroxides to the corresponding stable alcohols, by using glutathione as a source of reducing equivalents. It requires selenium as a cofactor and contains a selenocysteine amino acid residue in the active site of each monomer that participates in the actual mechanism of the enzyme. Glutathione Reductase (GR), provided with each kit, then reduces the oxidized glutathione to complete the cycle. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the absorbance at 340 nm is directly proportional to the Glutathione Peroxidase activity in the sample.
Other Resources
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Kit Contents for High Throughput Glutathione Peroxidase Assay Kit
- Glutathione Peroxidase
- 10X Assay Buffer
- GSH+NADPH
- Glutathione Reductase
- Cumene Hydroperoxide
- 96-well plates
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Product Documents for High Throughput Glutathione Peroxidase Assay Kit
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Citations for High Throughput Glutathione Peroxidase Assay Kit
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FAQs for High Throughput Glutathione Peroxidase Assay Kit
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A: This kit may be used to measure Glutathione Peroxidase activity in serum or plasma samples following the procedures in the kit insert.
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A:
The dynamic range of the Glutathione Peroxidase kit is dependent on source of the sample. The minimum level of sensitivity is twice that of the background slope of the change of absorbance at 340 nm versus time (in the absence of GP).
Some tissues may generate very high levels of GP and the sample will have to be serially diluted to give a good linear decrease in absorbance at 340 nm over a 10 minute period.