High Throughput Glutathione Peroxidase Assay Kit
High Throughput Glutathione Peroxidase Assay Kit Summary
• Suitable for a 96 well or cuvette format
• Suitable for plasma, erythrocyte lysates, tissue homogenates, and cell lysates
Why Use the HT Glutathione Peroxidase Assay Kit?
Tetrameric Glutathione Peroxidase catalyzes the reduction of H2O2 to water, and organic peroxides to the corresponding stable alcohols, by using glutathione as a source of reducing equivalents. It requires selenium as a cofactor and contains a selenocysteine amino acid residue in the active site of each monomer that participates in the actual mechanism of the enzyme. Glutathione Reductase (GR), provided with each kit, then reduces the oxidized glutathione to complete the cycle. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the absorbance at 340 nm is directly proportional to the Glutathione Peroxidase activity in the sample.
• Glutathione Peroxidase
• 10X Assay Buffer
• Glutathione Reductase
• Cumene Hydroperoxide
• 96-well plates
For research use only. Not for diagnositic use.
Citations for High Throughput Glutathione Peroxidase Assay Kit
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Citations: Showing 1 - 5
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Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway
Authors: XJ Chen, SM Ren, JZ Dong, CG Qiu, YW Chen, HL Tao
Drug Des Devel Ther, 2019;13(0):647-655. 2019
Dihydroartemisinin-induced unfolded protein response feedback attenuates ferroptosis via PERK/ATF4/HSPA5 pathway in glioma cells
Authors: Y Chen, Y Mi, X Zhang, Q Ma, Y Song, L Zhang, D Wang, J Xing, B Hou, H Li, H Jin, W Du, Z Zou
J. Exp. Clin. Cancer Res., 2019;38(1):402. 2019
Theaflavin attenuates cerebral ischemia/reperfusion injury by abolishing miRNA?128?3p?mediated Nrf2 inhibition and reducing oxidative stress
Authors: R Li, X Li, H Wu, Z Yang, L Fei, J Zhu
Mol Med Rep, 2019;0(0):. 2019
Glutathione-mediated detoxification of halobenzoquinone drinking water disinfection byproducts in T24 cells.
Authors: Li J, Wang W, Zhang H, Le X, Li X
Toxicol Sci, 0;141(2):335-43. 0
Genetic disruption of SOD1 gene causes glucose intolerance and impairs beta-cell function.
Authors: Muscogiuri G, Salmon A, Aguayo-Mazzucato C, Li M, Balas B, Guardado-Mendoza R, Giaccari A, Reddick R, Reyna S, Weir G, Defronzo R, Van Remmen H, Musi N
Diabetes, 0;62(12):4201-7. 0
Is this kit capable of measuring serum/plasma samples?
This kit may be used to measure Glutathione Peroxidase activity in serum or plasma samples following the procedures in the kit insert.
What is the dynamic range of this kit?
The dynamic range of the Glutathione Peroxidase kit is dependent on source of the sample. The minimum level of sensitivity is twice that of the background slope of the change of absorbance at 340 nm versus time (in the absence of GP).
Some tissues may generate very high levels of GP and the sample will have to be serially diluted to give a good linear decrease in absorbance at 340 nm over a 10 minute period.
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