High Throughput Glutathione Peroxidase Assay Kit
High Throughput Glutathione Peroxidase Assay Kit Summary
Key Benefits
• Suitable for a 96 well or cuvette format
• Suitable for plasma, erythrocyte lysates, tissue homogenates, and cell lysates
Why Use the HT Glutathione Peroxidase Assay Kit?
Tetrameric Glutathione Peroxidase catalyzes the reduction of H2O2 to water, and organic peroxides to the corresponding stable alcohols, by using glutathione as a source of reducing equivalents. It requires selenium as a cofactor and contains a selenocysteine amino acid residue in the active site of each monomer that participates in the actual mechanism of the enzyme. Glutathione Reductase (GR), provided with each kit, then reduces the oxidized glutathione to complete the cycle. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the absorbance at 340 nm is directly proportional to the Glutathione Peroxidase activity in the sample.
Other Resources
7512-100-k_cuvette_assay_calculation_worksheet7512-100-k_plate_assay_calculation_worksheet
Kit Contents
• Glutathione Peroxidase
• 10X Assay Buffer
• GSH+NADPH
• Glutathione Reductase
• Cumene Hydroperoxide
• 96-well plates
Specifications
Limitations
For research use only. Not for diagnositic use.
Product Datasheets
Citations for High Throughput Glutathione Peroxidase Assay Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway
Authors: XJ Chen, SM Ren, JZ Dong, CG Qiu, YW Chen, HL Tao
Drug Des Devel Ther, 2019;13(0):647-655. 2019
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Dihydroartemisinin-induced unfolded protein response feedback attenuates ferroptosis via PERK/ATF4/HSPA5 pathway in glioma cells
Authors: Y Chen, Y Mi, X Zhang, Q Ma, Y Song, L Zhang, D Wang, J Xing, B Hou, H Li, H Jin, W Du, Z Zou
J. Exp. Clin. Cancer Res., 2019;38(1):402. 2019
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Theaflavin attenuates cerebral ischemia/reperfusion injury by abolishing miRNA?128?3p?mediated Nrf2 inhibition and reducing oxidative stress
Authors: R Li, X Li, H Wu, Z Yang, L Fei, J Zhu
Mol Med Rep, 2019;0(0):. 2019
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Glutathione-mediated detoxification of halobenzoquinone drinking water disinfection byproducts in T24 cells.
Authors: Li J, Wang W, Zhang H, Le X, Li X
Toxicol Sci, 0;141(2):335-43. 0
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Genetic disruption of SOD1 gene causes glucose intolerance and impairs beta-cell function.
Authors: Muscogiuri G, Salmon A, Aguayo-Mazzucato C, Li M, Balas B, Guardado-Mendoza R, Giaccari A, Reddick R, Reyna S, Weir G, Defronzo R, Van Remmen H, Musi N
Diabetes, 0;62(12):4201-7. 0
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