High Throughput Glutathione Peroxidase Assay Kit

Quantification of glutathione peroxidase activity
Catalog # Availability Size / Price Qty
7512-100-K
Product Details
Citations (5)
FAQs
Reviews

High Throughput Glutathione Peroxidase Assay Kit Summary

Measures glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.

Key Benefits

• Suitable for a 96 well or cuvette format
• Suitable for plasma, erythrocyte lysates, tissue homogenates, and cell lysates

Why Use the HT Glutathione Peroxidase Assay Kit?

Tetrameric Glutathione Peroxidase catalyzes the reduction of H2O2 to water, and organic peroxides to the corresponding stable alcohols, by using glutathione as a source of reducing equivalents. It requires selenium as a cofactor and contains a selenocysteine amino acid residue in the active site of each monomer that participates in the actual mechanism of the enzyme. Glutathione Reductase (GR), provided with each kit, then reduces the oxidized glutathione to complete the cycle. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the absorbance at 340 nm is directly proportional to the Glutathione Peroxidase activity in the sample.

Other Resources

7512-100-k_cuvette_assay_calculation_worksheet7512-100-k_plate_assay_calculation_worksheet

Kit Contents

• Glutathione Peroxidase
• 10X Assay Buffer
• GSH+NADPH
• Glutathione Reductase
• Cumene Hydroperoxide
• 96-well plates

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Citations for High Throughput Glutathione Peroxidase Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway
    Authors: XJ Chen, SM Ren, JZ Dong, CG Qiu, YW Chen, HL Tao
    Drug Des Devel Ther, 2019;13(0):647-655.  2019
  2. Dihydroartemisinin-induced unfolded protein response feedback attenuates ferroptosis via PERK/ATF4/HSPA5 pathway in glioma cells
    Authors: Y Chen, Y Mi, X Zhang, Q Ma, Y Song, L Zhang, D Wang, J Xing, B Hou, H Li, H Jin, W Du, Z Zou
    J. Exp. Clin. Cancer Res., 2019;38(1):402.  2019
  3. Theaflavin attenuates cerebral ischemia/reperfusion injury by abolishing miRNA?128?3p?mediated Nrf2 inhibition and reducing oxidative stress
    Authors: R Li, X Li, H Wu, Z Yang, L Fei, J Zhu
    Mol Med Rep, 2019;0(0):.  2019
  4. Glutathione-mediated detoxification of halobenzoquinone drinking water disinfection byproducts in T24 cells.
    Authors: Li J, Wang W, Zhang H, Le X, Li X
    Toxicol Sci, 0;141(2):335-43.  0
  5. Genetic disruption of SOD1 gene causes glucose intolerance and impairs beta-cell function.
    Authors: Muscogiuri G, Salmon A, Aguayo-Mazzucato C, Li M, Balas B, Guardado-Mendoza R, Giaccari A, Reddick R, Reyna S, Weir G, Defronzo R, Van Remmen H, Musi N
    Diabetes, 0;62(12):4201-7.  0

FAQs

  1. Is this kit capable of measuring serum/plasma samples? 

    • This kit may be used to measure Glutathione Peroxidase activity in serum or plasma samples following the procedures in the kit insert.

  2. What is the dynamic range of this kit?



    • The dynamic range of the Glutathione Peroxidase kit is dependent on source of the sample. The minimum level of sensitivity is twice that of the background slope of the change of absorbance at 340 nm versus time (in the absence of GP).

      Some tissues may generate very high levels of GP and the sample will have to be serially diluted to give a good linear decrease in absorbance at 340 nm over a 10 minute period.

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