Hip Antibody (5D11) - Azide and BSA Free

Novus Biologicals | Catalog # H00064399-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5D11

Format

Azide and BSA Free
View all Hip Primary Antibodies »

Product Specifications

Immunogen

HHIP (NP_071920, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSPHSQ

Reactivity Notes

Other species not tested.

Specificity

HHIP - hedgehog interacting protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Hip Antibody (5D11) - Azide and BSA Free

Western Blot: Hip Antibody (5D11) [H00064399-M01]

Western Blot: Hip Antibody (5D11) [H00064399-M01]

Western Blot: Hip Antibody (5D11) [H00064399-M01] - HHIP monoclonal antibody (M01), clone 5D11. Analysis of HHIP expression in MCF-7.
Immunohistochemistry-Paraffin: Hip Antibody (5D11) [H00064399-M01]

Immunohistochemistry-Paraffin: Hip Antibody (5D11) [H00064399-M01]

Immunohistochemistry-Paraffin: Hip Antibody (5D11) [H00064399-M01] - Analysis of monoclonal antibody to HHIP on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.
Western Blot: Hip Antibody (5D11) [H00064399-M01]

Western Blot: Hip Antibody (5D11) [H00064399-M01]

Western Blot: Hip Antibody (5D11) [H00064399-M01] - HHIP monoclonal antibody (M01), clone 5D11 Analysis of HHIP expression in Hela S3 NE.
Western Blot: Hip Antibody (5D11) [H00064399-M01]

Western Blot: Hip Antibody (5D11) [H00064399-M01]

Western Blot: Hip Antibody (5D11) [H00064399-M01] - Analysis of HHIP expression in transfected 293T cell line by HHIP monoclonal antibody (M01), clone 5D11.Lane 1: HHIP transfected lysate(78.9 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: Hip Antibody (5D11) [H00064399-M01]

Immunoprecipitation: Hip Antibody (5D11) [H00064399-M01]

Immunoprecipitation: Hip Antibody (5D11) [H00064399-M01] - HHIP transfected lysate using anti-HHIP monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HHIP MaxPab rabbit polyclonal antibody.
Immunoprecipitation: Hip Antibody (5D11) [H00064399-M01]

Immunoprecipitation: Hip Antibody (5D11) [H00064399-M01]

Immunoprecipitation: Hip Antibody (5D11) [H00064399-M01] - Analysis of HHIP transfected lysate using anti-HHIP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HHIP MaxPab rabbit polyclonal antibody.
ELISA: Hip Antibody (5D11) [H00064399-M01]

ELISA: Hip Antibody (5D11) [H00064399-M01]

ELISA: Hip Antibody (5D11) [H00064399-M01] - Detection limit for recombinant GST tagged HHIP is approximately 1ng/ml as a capture antibody.

Applications for Hip Antibody (5D11) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Hip

This gene encodes a protein similar to the mouse hedgehog-interacting protein, a regulatory component of the hedgehog signalling pathway. Members of the hedgehog family are evolutionarily conserved proteins which are involved in many fundamental processes in embryonic development, including anteroposterior patterns of limbs and regulation of left-right asymmetry.

Long Name

Hedgehog-interacting Protein

Alternate Names

HHIP

Entrez Gene IDs

64399 (Human)

Gene Symbol

HHIP

OMIM

606178 (Human)

UniProt

NP_071920

Additional Hip Products

Product Documents for Hip Antibody (5D11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Hip Antibody (5D11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Hip Antibody (5D11) - Azide and BSA Free

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Protocols

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FAQs

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