Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-03981

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone Deacetylase 4/HDAC4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human HDAC4 around amino acids 630~634 (A-Q-S-S-P).

Specificity

Detects endogenous level of total Histone Deacetylase 4/HDAC4 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-03981]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-03981]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-03981] - Western blot analysis of extracts from HepG2 cells using NBP2-03981
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-03981]

Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-03981]

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-03981] - mmunofluorescence staining of methanol-fixed Hela cells using NBP2-03981
Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Western Blot: Histone Deacetylase 4/HDAC4 Antibody - BSA Free [NBP2-03981] -

Western Blot: Histone Deacetylase 4/HDAC4 Antibody - BSA Free [NBP2-03981] - Western blotting analysis using NBP2-03981.

Applications for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 4/HDAC4

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past several years. These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns. These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Long Name

Histone Deacetylase 4

Alternate Names

HDAC4

Gene Symbol

HDAC4

UniProt

NP_848510 (Human)

Additional Histone Deacetylase 4/HDAC4 Products

Product Documents for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adult Neurogenesis
Epigenetics

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Protocols

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