Histone H3 [Monomethyl Lys9] Antibody - BSA Free

Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.

Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - ChIP assays were performed using human HeLa cells, the antibody directed against H3K9me1 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 10,000 cells. Respectively 1 and 5 ug of the antibody and 5 ug of IgG (negative IP control) were used per ChIP experiment. QPCR was performed with primers for the GAPDH promoter and for the inactive gene MYOD. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K9me1 is preferably present at silent genes.

Dot Blot: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K9me1 with peptides containing other modifications and the unmodified sequence of histone H3. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.