Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-16137

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Histone H4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic lactylated peptide around K5 of human Histone H4 (NP_003539.1). GRGKG

Reactivity Notes

(Wide Range Predicted)

Modification

Lactyl Lys5

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free

Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free

Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] -

Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from NIH/3T3 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Sodium lactate(100mM) for 24h.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free

Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] -

Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from C6 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. C6 cells were treated by Sodium lactate(100mM) for 24h.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free

Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] -

Western Blot: Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free [NBP3-16137] - Western blot analysis of lysates from MCF7 cells, using Histone H4 Rabbit pAb at 1:1000 dilution. MCF7 cells were treated by Sodium lactate(100mM) for 24h.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Dot Blot: Histone H4 [Lactyl Lys5] Antibody [NBP3-16137] - Dot-blot analysis of all sorts of peptides using Histone H4 antibody (NBP3-16137) at 1:4000 dilution.

Applications for Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free

Application
Recommended Usage

Dot Blot

1:500 - 1:4000

ELISA

Recommended starting concentration is 1 μg/mL.

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H4

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4

Gene Symbol

H4C16

Additional Histone H4 Products

Product Documents for Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Histone H4 [Lactyl Lys5] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Epigenetics
Notch Pathway

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail