A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 155-321 of HIV-1 Gag p24

Source:E.coli

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MWVKVVEEKA FSPEVIPMFS ALSEGATPQD LNTMLNTVGG HQAAMQMLKE TINEEAAEWD RLHPVHAGPI APGQMREPRG SDIAGTTSTL QEQIGWMTHN PPIPVGEIYK RWIILGLNKI VRMYSPTSIL DIRQGPKEPF RDYVDRFYKT LRAEQASQEV KNWMTETL

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

21.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant HIV-1 Gag p24 His Protein

SDS-PAGE: Recombinant HIV-1 Gag p24 His Protein [NBP1-46036]

SDS-Page: Recombinant Virus HIV-1 Gag p24 His Protein [NBP1-46036] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation, and Storage

NBP1-46036

Formulation	20 mM Tris-HCl buffer (pH 8.0), 100 mM NaCl, 0.1 mM PMSF, 10% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIV-1 Gag p24

The HIV1 p24 protein performs highly complex orchestrated tasks during the assembly, budding maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag p24, the major capsid protein of the HIV-1 virion, has been used in clinical trials as one of the components of the HIV-1 vaccine because of the high degree of sequence conservation between different isolates. Recombinant HIV1-p24 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Alternate Names

CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)

Gene Symbol

gag

Additional HIV-1 Gag p24 Products

Product Documents for Recombinant HIV-1 Gag p24 His Protein

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Product Specific Notices for Recombinant HIV-1 Gag p24 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Infectious Diseases

Citations for Recombinant HIV-1 Gag p24 His Protein

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Recombinant HIV-1 Gag p24 His Protein

Novus Biologicals | Catalog # NBP1-46036

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