Recombinant Human HLA DOA His Protein

Name: Recombinant Human HLA DOA His Protein
Brand: Novus Biologicals
SKU: NBP2-23211
Price: 329 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-23211

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all HLA DOA Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 26-217 of Human HLA DOA

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSTKADHMG SYGPAFYQSY GASGQFTHEF DEEQLFSVDL KKSEAVWRLP EFGDFARFDP QGGLAGIAAI KAHLDILVER SNRSRAINVP PRVTVLPKSR VELGQPNILI CIVDNIFPPV INITWLRNGQ TVTEGVAQTS FYSQPDHLFR KFHYLPFVPS AEDVYDCQVE HWGLDAPLLR HWELQVPIPP PDAME

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

24.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human HLA DOA His Protein

SDS-PAGE: Recombinant Human HLA DOA His Protein [NBP2-23211] -

SDS-Page: HLA DOA Protein [NBP2-23211] - 3ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.

Formulation, Preparation, and Storage

NBP2-23211

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLA DOA

HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. Recombinant human HLA-DAO protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

HLA class II histocompatibility antigen, DO alpha chain, HLA-D0-alpha, HLA-DNAHLADZ, HLA-DZA, lymphocyte antigen, major histocompatibility complex, class II, DN alpha, major histocompatibility complex, class II, DO alpha, MHC class II antigen DOA, MHC DN-alpha, MHC DZ alpha

Gene Symbol

HLA-DOA

Additional HLA DOA Products

Product Documents for Recombinant Human HLA DOA His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human HLA DOA His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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