HLA DQB1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00003119-B01P

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Format

Azide and BSA Free
View all HLA DQB1 Primary Antibodies »

Product Specifications

Immunogen

HLA-DQB1 (AAH12106.1, 1 a.a. - 261 a.a.) full-length human protein. MSWKKALRIPGGLRVATVTLMLAMLSTPVAEGRDSPEDFVYQFKGMCYFTNGTERVRLVTRYIYNREEYARFDSDVGVYRAVTPLGPPAAEYWNSQKEVLERTRAELDTVCRHNYQLELRTTLQRRVEPTVTISPSRTEALNHHNLLVCSVTDFYPAQIKVRWFRNDQEETTGVVSTPLIRNGDWTFQILVMLEMTPQRGDVYTCHVEHPSLQNPIIVEWRAQSESAQSKMLSGIGGFVLGLIFLGLGLIIHHRSQKGLLH

Specificity

HLA-DQB1 - major histocompatibility complex, class II, DQ beta 1,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for HLA DQB1 Antibody - Azide and BSA Free

Western Blot: HLA DQB1 Antibody [H00003119-B01P]

Western Blot: HLA DQB1 Antibody [H00003119-B01P]

Western Blot: HLA DQB1 Antibody [H00003119-B01P] - Analysis of HLA-DQB1 expression in human pancreas.
Flow Cytometry: HLA DQB1 Antibody [H00003119-B01P]

Flow Cytometry: HLA DQB1 Antibody [H00003119-B01P]

Flow Cytometry: HLA DQB1 Antibody [H00003119-B01P] - Analysis of negative control 293 cells (Black) and HLA-DQB1 expressing 293 cells (Green) using HLA-DQB1 purified mouse polyclonal antibody.
Western Blot: HLA DQB1 Antibody [H00003119-B01P]

Western Blot: HLA DQB1 Antibody [H00003119-B01P]

Western Blot: HLA DQB1 Antibody [H00003119-B01P] - Analysis of HLA-DQB1 expression in transfected 293T cell line by HLA-DQB1 polyclonal antibody. Lane 1: HLA-DQB1 transfected lysate(28.71 KDa). Lane 2: Non-transfected lysate.

Applications for HLA DQB1 Antibody - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been use for FACS.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HLA DQB1

HLA-DQB1 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq]

Alternate Names

DC-1 alpha chain, DC-alpha, HLA class II histocompatibility antigen, DQ alpha 1 chain-like, HLA-DCA, MHC class II DQA1

Entrez Gene IDs

3119 (Human)

Gene Symbol

HLA-DQB1

UniProt

AAH12106.1 (Human)

Additional HLA DQB1 Products

Product Documents for HLA DQB1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HLA DQB1 Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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