HLA DRB4 Antibody (3Z5V9)

Novus Biologicals | Catalog # NBP3-15265

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3Z5V9 expressed in HEK293
View all HLA DRB4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human HLA DRB4 (P13762). TERVWNLIRYIYNQEEYARYNSDLGEYQAVTELGRPDAEYWNSQKDLLERRRAEVDTYCRYNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVNG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HLA DRB4 Antibody (3Z5V9)

Western Blot: HLA DRB4 Antibody (3Z5V9) [NBP3-15265]

Western Blot: HLA DRB4 Antibody (3Z5V9) [NBP3-15265]

Western Blot: HLA DRB4 Antibody (3Z5V9) [NBP3-15265] - Western blot analysis of extracts of Raji cells, using HLA DRB4 antibody (NBP3-15265) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for HLA DRB4 Antibody (3Z5V9)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HLA DRB4

HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

Alternate Names

class II histocompatibility antigen HLA DR alpha, beta1-0307, DR12, DR-12, DR13, DR-13, DR14, DR-14, DR4, DR-4, DRB1 transplantation antigen, DRB4, HLA class II histocompatibility antigen, DR beta 4 chain, HLA class II histocompatibility antigen, DRB1-12 beta chain, HLA class II histocompatibility antigen, DRB1-4 beta chain, HLA-DR4B, human leucocyte antigen DRB4, leucocyte antigen DRB1, leukocyte antigen, major histocompatibility complex, class II, DR beta 1, major histocompatibility complex, class II, DR beta 4, MHC class I antigen DRB1*12, MHC class I antigen DRB1*4, MHC class II antigen DRB1*12, MHC class II antigen DRB1*13, MHC class II antigen DRB1*14, MHC class II antigen DRB1*4, MHC class II antigen DRB4, MHC class II antigen HLA-DRB1, MHC class II antigen HLA-DR-beta, MHC class II HLA-DR-beta-7, MHC class2 antigen, MHC HLA DR-beta chain

Gene Symbol

HLA-DRB4

Additional HLA DRB4 Products

Product Documents for HLA DRB4 Antibody (3Z5V9)

Certificate of Analysis

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Product Specific Notices for HLA DRB4 Antibody (3Z5V9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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