HLA DRB5 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00003127-D01P

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all HLA DRB5 Primary Antibodies »

Product Specifications

Immunogen

HLA-DRB5 (NP_002116.2, 1 a.a. - 266 a.a.) full-length human protein. MVCLKLPGGSYMAKLTVTLMVLSSPLALAGDTRPRFLQQDKYECHFFNGTERVRFLHRDIYNQEEDLRFDSDVGEYRAVTELGRPDAEYWNSQKDFLEDRRAAVDTYCRHNYGVGESFTVQRRVEPKVTVYPARTQTLQHHNLLVCSVNGFYPGSIEVRWFRNSQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRAQSESAQSKMLSGVGGFVLGLLFLGAGLFIYFKNQKGHSGLHPTGLVS

Specificity

Reacts with major histocompatibility complex, class II, DR beta 5.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for HLA DRB5 Antibody - Azide and BSA Free

Western Blot: HLA DRB5 Antibody [H00003127-D01P]

Western Blot: HLA DRB5 Antibody [H00003127-D01P]

Western Blot: HLA DRB5 Antibody [H00003127-D01P] - Analysis of HLA-DRB5 expression in human kidney.
Western Blot: HLA DRB5 Antibody [H00003127-D01P]

Western Blot: HLA DRB5 Antibody [H00003127-D01P]

Western Blot: HLA DRB5 Antibody [H00003127-D01P] - Analysis of HLA-DRB5 expression in HeLa.
Western Blot: HLA DRB5 Antibody [H00003127-D01P]

Western Blot: HLA DRB5 Antibody [H00003127-D01P]

Western Blot: HLA DRB5 Antibody [H00003127-D01P] - Analysis of HLA-DRB5 expression in transfected 293T cell line by HLA-DRB5 polyclonal antibody.Lane 1: HLA-DRB5 transfected lysate(30.10 KDa).Lane 2: Non-transfected lysate.

Applications for HLA DRB5 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against tissue and transfected lysate in WB and as a detection antibody in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HLA DRB5

HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq]

Alternate Names

DR beta-5, DR2-beta-2, DR9, DR-9, Dw2, FLJ76359, HLA class II histocompatibility antigen, DR beta 5 chain, HLA class II histocompatibility antigen, DR-5 beta chain, HLA class II histocompatibility antigen, DRB1-9 beta chain, HLA-DRB, human leucocyte antigen DRB5, leukocyte antigen class II, major histocompatibility complex, class II, DR beta 5, MHC class II antigen DRB1*9, MHC class II antigen DRB5, MHC class II HLA beta chain, MHC HLA-DR-beta cell surface glycoprotein, MHC HLA-DR-beta chain

Entrez Gene IDs

3127 (Human)

Gene Symbol

HLA-DRB5

UniProt

NP_002116.2 (Human)

Additional HLA DRB5 Products

Product Documents for HLA DRB5 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HLA DRB5 Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for HLA DRB5 Antibody - Azide and BSA Free

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  • Q: We are interested in custom labeling our antibodies to be able to integrate new markers into already existing multicolor FACS panels. We would be glad to receive pricing and general order information for anti-HLA-DRB1 and anti-HLA-DRB5. To estimate expenses for future antibody labeling orders it would be helpful to know on what basis (amount, type, etc.) the price is going to be calculated. Right now the antibodies are still in mouse ascites. Would it be possible to integrate the elution of the antibodies from the ascites before labeling as part of the service agreement?

    A:

    While we do not generally do this kind of custom purification unless it is for very large amounts of antibody, we do have a number of kits available that may be of use to you. Since an antibody that is still in ascites can not be labeled, it will need to be purified. A list of our kits available for this can be found at the following link: AbSelect. Once the antibodies are purified, they can be labeled with any number of different colors. A list of these kits for labeling can be found at the following link: Lightning-Link.

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