Human HLA G ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-62174

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

EDTA plasma, amniotic fluid, cell culture supernatant (100 uL)

Sensitivity

0.6U/ml

Assay Range

3.91 to 125U/ml
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

Detects soluble human HLA-G (leukocyte antigen-G) in EDTA plasma, amniotic fluid, and cell culture supernatant. Cross-reacts strongly with bovine, dog, goat, monkey, rabbit and sheep HLA-G. Cross-reacts weakly with cat HLA-G.

Precision

Intra-Assay Precision (Precision within an assay) Low Concentration %CV - 4.9% High - 3.5%

Inter-Assay Precision (Precision between assays) Low Concentration %CV - 6.5% High - 7.4%

Recovery for Human HLA G ELISA Kit (Colorimetric)

Recovery

Low 1 - 94.8%
Low 2 - 88.2%
Low 3 - 81.7%

High 1 - 96.1%
High 2 - 95.8%
High 3 - 90.4%

Scientific Data Images for Human HLA G ELISA Kit (Colorimetric)

ELISA: Human HLA G ELISA Kit (Colorimetric) [NBP2-62174]

ELISA: Human HLA G ELISA Kit (Colorimetric) [NBP2-62174]

ELISA: Human HLA G ELISA Kit (Colorimetric) [NBP2-62174] - Example standard curve for Human HLA G ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Human HLA G ELISA Kit (Colorimetric)

  • Conjugate
  • Conjugate Diluent
  • Dilution Buffer 1
  • Dilution Buffer 2
  • Master Calibrator
  • Plate
  • Stop Solution
  • Substrate Solution
  • Wash Solution (10X)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: HLA-G

Human leukocyte antigen (HLA)-G is a non-classical major histocompatibility complex (MHC) class I gene belonging to the HLA class I family. The HLA class I family is subdivided into the classical (HLA-Ia) antigens comprised of HLA-A, -B, and -C molecules and the nonclassical (HLA-Ib) group including the HLA-E, -F, and -G molecules. Seven different splice variants of HLA-G have been observed including four membrane isoforms (HLA-G1, HLA-G2, HLA-G3, and HLA-G4) and three soluble isoforms (HLA-G5, HLA-G6 and HLA-G7). Proteolytic processing of HLA-G1 by metalloproteinases (MMPs) such as MMP-2 produces the soluble isoform, shedding HLA-G1. The HLA-G1 and HLA-G5 isoforms share a similar confirmation to the classical MHC class I protein, consisting of three extracellular domains including alpha1, alpha2, and alpha3 associated with Beta-2-microglobulin (B2M). Other HLA-G variants are shorter, missing one or two of the globular domains and are not bound to B2M. HLA-G receptors include KIR2DL4 (CD158d), with the highest affinity for ILT2 (LILRB1, CD85j) and ILT4 (LILRB2, CD85d) (1,2).

Discovered in cytotrophoblasts, HLA-G is involved in fetal maternal immune tolerance and some studies have linked its downregulation with severe preeclampsia (3). HLA-G mediated immune suppression works by impeding cell proliferation, differentiation, cytotoxicity, cytokine secretion and chemotaxis; and activation of regulatory cells and MDSCs or M2 type macrophage. HLA-G is constitutively expressed in immune-privileged sites such as pancreatic islets, mesenchymal stem cells (MSCs) and the cornea. Upregulation of HLA-G occurs in pathological states including cancer, allo-transplantations, viral infections, autoimmune and inflammatory diseases (4). In cancer, HLA-G expression is induced by hypoxia and has been correlated with advanced tumor stage, tumor metastasis, and poor therapeutic response and survival. HLA-G is an attractive tumor associated-antigen (TAA) for immunotherapy and is considered a major immune checkpoint (ICP).

References

1. Loustau, M., Anna, F., Drean, R., Lecomte, M., Langlade-Demoyen, P., & Caumartin, J. (2020). HLA-G Neo-Expression on Tumors. Front Immunol, 11:1685. PMID: 32922387

2. Lin A, Yan WH. (2018) Heterogeneity of HLA-G Expression in Cancers: Facing the Challenges. Front Immunol. 9:2164. PMID: 30319626

3. Djurisic S, Hviid TV. (2014) HLA Class Ib Molecules and Immune Cells in Pregnancy and Preeclampsia. Front Immunol. 5:652. PMID: 25566263

4. Lila N, Rouas-Freiss N, Dausset J, Carpentier A, Carosella ED. (2001) Soluble HLA-G protein secreted by allo-specific CD4+ T cells suppresses the allo-proliferative response: a CD4+ T cell regulatory mechanism. Proc Natl Acad Sci U S A. 98(21):12150-12155. PMID: 11572934

Long Name

Major Histocompatibility Complex, Class I, G

Alternate Names

HLA G, HLA-6.0, HLAG, MHC Class I Antigen G, MHC-G

Gene Symbol

HLA-G

Additional HLA-G Products

Product Documents for Human HLA G ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human HLA G ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Human HLA G ELISA Kit (Colorimetric)

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FAQs for Human HLA G ELISA Kit (Colorimetric)

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  • Q: Does this ELISA measure HLA-G1, HLA-G5, or both?

    A: This Human HLA G ELISA Kit (NBP2-62174) measures shedded HLA-G1 and HLA-G5 in serum, plasma, amniotic fluid or cell culture supernatant

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