HMGB1/HMG-1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-27169

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (93%). Backed by our 100% Guarantee.

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all HMGB1/HMG-1 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 25-94 of human HMGB1 was used as the immunogen.

Reactivity Notes

The HMGB1 amino acid sequence used as immunogen is 100% homologous in rat, and monkey.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HMGB1/HMG-1 Antibody - Azide and BSA Free

Western Blot: HMGB1/HMG-1 Antibody [NBP2-27169]

Western Blot: HMGB1/HMG-1 Antibody [NBP2-27169]

Western Blot: HMGB1/HMG-1 Antibody [NBP2-27169] - Analysis using HMGB1 antibody. Mouse NIH 3T3 cell lysate was probed with HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Simple Western: HMGB1/HMG-1 Antibody [NBP2-27169]

Simple Western: HMGB1/HMG-1 Antibody [NBP2-27169]

Simple Western: HMGB1/HMG-1 Antibody [NBP2-27169] - Simple Western lane view shows a specific band for HMGB1 in 0.2 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for HMGB1/HMG-1 Antibody - Azide and BSA Free

Application
Recommended Usage

Simple Western

1:200

Western Blot

5-10ug/ml
Application Notes
Minimal staining in IHC and ELISPOT indicates that this antibody preferentially detects the reduced form of HMGB1.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat lysate 0.2 mg/mL, separated by Size, antibody dilution of 1:200, apparent MW was 36 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

HMGB1 (high mobility group protein B1) belongs to the HMGB family and contains two HMG box DNA-binding domains. It is a highly conserved, ubiquitous protein present in the nuclei and cytoplasm of nearly all cell types, and is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). It is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HMGB1/HMG-1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for HMGB1/HMG-1 Antibody - Azide and BSA Free

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  • Q: Are there any referencing publications for this product?

    A: Unfortunately, we do not have any publications referenced for this antibody. The only available validation information for WB is what is on the website/datasheet. 

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