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Human HMGB1/HMG-1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-62766

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV 5.09

Inter-Assay Precision (Precision between assays) %CV 4.52

Recovery for Human HMGB1/HMG-1 ELISA Kit (Colorimetric)

Recovery

93 - 108%

Linearity

Human HMGB1/HMG-1 ELISA Kit (Colorimetric)

Scientific Data Images for Human HMGB1/HMG-1 ELISA Kit (Colorimetric)

Human HMGB1/HMG-1 ELISA Kit (Colorimetric)

ELISA: Human HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62766] -

ELISA: Human HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62766] - Standard Curve Reference
Human HMGB1/HMG-1 ELISA Kit (Colorimetric)

ELISA: Human HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62766]

HMGB1 concentrations for untreated cells compared to four treatments, showing a decrease in secreted HMGB1 with all treatments. Image from a verified customer review.

Kit Contents for Human HMGB1/HMG-1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HMGB1/HMG-1

HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for Human HMGB1/HMG-1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human HMGB1/HMG-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Human HMGB1/HMG-1 ELISA Kit (Colorimetric)

Customer Reviews for Human HMGB1/HMG-1 ELISA Kit (Colorimetric) (2)

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  • Human HMGB1/HMG-1 ELISA Kit (Colorimetric)
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 06/26/2025
    HMGB1 concentrations for untreated cells compared to four treatments, showing a decrease in secreted HMGB1 with all treatments.
    Human HMGB1/HMG-1 ELISA Kit (Colorimetric) NBP2-62766
  • Human HMGB1/HMG-1 ELISA Kit (Colorimetric)
    Name: Xuanrong Chen
    Sample Tested: Blood mononuclear cells (PBMCs)
    Verified Customer | Posted 07/02/2022

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FAQs for Human HMGB1/HMG-1 ELISA Kit (Colorimetric)

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  • Q: Can we use the standard material in this HMGB1/HMG-1 ELISA kit past the 6 month expiration date?

    A: Our products do not have a set expiration date, but we guarantee the performance for 6 months if stored properly. If stored properly, the kit will most likely still work past the guarantee date.

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