Recombinant Human HMGB2 His Protein

Novus Biologicals | Catalog # NBP1-72386

Novus Biologicals
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Key Product Details

Source

Baculovirus

Tag

His

Applications

SDS-PAGE
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Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-209 of Human HMGB2

Source: Baculovirus

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH TGSMGKGDPN KPRGKMSSYA FFVQTCREEH KKKHPDSSVN FAEFSKKCSE RWKTMSAKEK SKFEDMAKSD KARYDREMKN YVPPKGDKKG KKKDPNAPKR PPSAFFLFCS EHRPKIKSEH PGLSIGDTAK KLGEMWSEQS AKDKQPYEQK AAKLKEKYEK DIAAYRAKGK SEAGKKGPGR PTGSKKKNEP EDEEEEEEEE DEDEEEEDED EE

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

26.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human HMGB2 His Protein

SDS-PAGE: Recombinant Human HMGB2 His Protein [NBP1-72386]

SDS-PAGE: Recombinant Human HMGB2 His Protein [NBP1-72386]

SDS-Page: Recombinant Human HMGB2 Protein [NBP1-72386] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBP1-72386
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 30% glycerol, 0.1 M NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGB2

High mobility group protein B2, also known as HMGB2, is a member of the non-histone chromosomal high-mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. Recombinant human HMGB2 protein, fused to His-tag at N-terminus, was expressed in Baculovirus and purified by using conventional chromatography techniques.

Alternate Names

High mobility group protein 2, high mobility group protein B2, high-mobility group box 2, HMG-2, HMG2high-mobility group (nonhistone chromosomal) protein 2

Gene Symbol

HMGB2

Additional HMGB2 Products

Product Documents for Recombinant Human HMGB2 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human HMGB2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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