HNF-4 gamma/NR2A2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-98899

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HNF-4 gamma/NR2A2 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Mouse HNF-4 gamma/NR2A2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for HNF-4 gamma/NR2A2 Antibody - BSA Free

Western Blot: HNF-4 gamma/NR2A2 Antibody [NBP2-98899]

Western Blot: HNF-4 gamma/NR2A2 Antibody [NBP2-98899]

Western Blot: HNF-4 gamma/NR2A2 Antibody [NBP2-98899] - Anti-HNF-4 gamma/NR2A2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse stomach tissue Lysate. Lane B: Mouse spleen tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 45 kDa
Immunoprecipitation: HNF-4 gamma/NR2A2 Antibody [NBP2-98899]

Immunoprecipitation: HNF-4 gamma/NR2A2 Antibody [NBP2-98899]

Immunoprecipitation: HNF-4 gamma/NR2A2 Antibody [NBP2-98899] - HNF-4 gamma/NR2A2 was immunoprecipitated using: Lane A: 0.5 mg mouse spleen tissue Lysate 4 ul anti-HNF-4 gamma/NR2A2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-HNF-4 gamma/NR2A2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 46 kDa

Applications for HNF-4 gamma/NR2A2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HNF-4 gamma/NR2A2

Hepatocyte nuclear factor 4 gamma (HNF4 gamma) is a NR2 Hepatocyte NF4-Like receptor of relatively unknown function. Although HNF4 gamma is structurally related to HNF4 alpha and is expressed together with HNF 4 Alpha in pancreatic islets, it does not play a major role in the etiology of early onset, autosomal dominant, non insulin-dependent type 2 diabetes or maturity-onset diabetes of the young, MODY1. A pseudogene of HNF4 gamma, located on chromosome 13 (13q14.11 - 13q14.3), has been recently identified using a comparative genomics approach. HNF4 gamma expression has been reported human pancreas, kidney, testis, small intestine, and colon. ESTs have been isolated from human tissue libraries, including cancerous brain and colon, and normal brain,stomach, and liver.

Long Name

Hepatocyte Nuclear Factor 4 gamma

Alternate Names

HNF4 gamma, HNF4G, NR2A2

Gene Symbol

HNF4G

Additional HNF-4 gamma/NR2A2 Products

Product Documents for HNF-4 gamma/NR2A2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HNF-4 gamma/NR2A2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail