Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-49782

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.1 ng/mL

Assay Range

0.16 - 10 ng/mL
View all HO-1/HMOX1/HSP32 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat HO-1/HMOX1/HSP32 in samples. No significant cross-reactivity or interference between Rat HO-1/HMOX1/HSP32 and analogues was observed

Description

Assay Length: 3 h 30 min

Precision

Intra-Assay Precision (Precision within an assay) CV% 10

Inter-Assay Precision (Precision between assays) CV% 10

Recovery for Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

Recovery

80 - 120%

Linearity

Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)
Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

Scientific Data Images for Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

ELISA: Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric) [NBP3-49782]

Standard Curve Reference.

Kit Contents for Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HO-1/HMOX1/HSP32

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Long Name

Heme Oxygenase 1

Alternate Names

HMOX1, HO1, HSP32

Gene Symbol

HMOX1

Additional HO-1/HMOX1/HSP32 Products

Product Documents for Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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