Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride
Biological ActivityHoechst 33342 stains and binds minor groove of AT-rich regions. Used to quantify DNA in viable cells. Cell permeable fluorescent DNA dye.
Application of Hoechst 33342 in HeLa cells. Fluorescence microscopy image of GLUT4-EGFP HeLa cells treated with Hoechst 33342. Colors: green (GLUT4-EGFP), red (SCOTfluor glucose probe 510 (100 μm); Catalog # 7447), blue (Hoechst 33342). Image kindly provided by Dr Sam Benson, Centre for Inflammation Research, University of Edinburgh.
Optical Data for Hoechst 33342
|Application||Flow Cytometry, Confocal imaging|
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Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study.
Portugal and Waring
Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter.
Citations for Hoechst 33342
The citations listed below are publications that use Tocris products. Selected citations for Hoechst 33342 include:
3 Citations: Showing 1 - 3
SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance.
Authors: Yang Et al.
Subcutaneous Inoculation of 3D Pancreatic Cancer Spheroids Results in Development of Reproducible Stroma-Rich Tumors.
Authors: Durymanov Et al.
Transl Oncol 2019;12:180
Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing.
Authors: Tabdanov Et al.
Cell Rep 2018;25:328
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Reviews for Hoechst 33342
Average Rating: 5 (Based on 3 Reviews)
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Hoechst was used for nuclear staining. Add stain at 1 ug/mL and incubate cells at room temperature for 10 minutes.
I used this dye to stain the nucleus of live cells. After making the stock solution, diluted the dye 1:1000 ratio in the culture media/PBS and added to cells and incubated for 5 mins.