Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride
Biological ActivityCell permeable fluorescent DNA stain; binds minor groove of AT-rich regions. Used to quantify DNA in viable cells.
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Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study.
Portugal and Waring
Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter.
Citations for Hoechst 33342
The citations listed below are publications that use Tocris products. Selected citations for Hoechst 33342 include:
3 Citations: Showing 1 - 3
SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance.
Authors: Yang Et al.
Subcutaneous Inoculation of 3D Pancreatic Cancer Spheroids Results in Development of Reproducible Stroma-Rich Tumors.
Authors: Durymanov Et al.
Transl Oncol 2019;12:180
Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing.
Authors: Tabdanov Et al.
Cell Rep 2018;25:328
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Average Rating: 5 (Based on 2 Reviews)
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Add Hoechst dyes at 1 ug/mL.Incubate cells at room temperature for 10 minutes.
Immunostaining of HEK 293 cells