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Hoechst 33342

Catalog #: 5117
3 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5117/50
Hoechst 33342 | CAS No. 875756-97-1 | Fluorescent DNA Stains
1 Image
Description: Fluorescent dye for staining DNA

Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride

Purity: ≥98%

Product Details
Citations (3)
Reviews (3)
Biological Activity Scientific Data Technical Data Background Product Datasheets Calculators

Biological Activity

Hoechst 33342 stains and binds minor groove of AT-rich regions. Used to quantify DNA in viable cells. Cell permeable fluorescent DNA dye.

Scientific Data

Fluorescence Imaging Hoechst 33342 | CAS No. 875756-97-1 | Fluorescent dye for staining DNA View Larger

Application of Hoechst 33342 in HeLa cells. Fluorescence microscopy image of GLUT4-EGFP HeLa cells treated with Hoechst 33342. Colors: green (GLUT4-EGFP), red (SCOTfluor glucose probe 510 (100 μm); Catalog # 7447), blue (Hoechst 33342). Image kindly provided by Dr Sam Benson, Centre for Inflammation Research, University of Edinburgh.

Optical Data for Hoechst 33342

λabs 350 nm
λem 461 nm
Cell Permeable Yes
Application Flow Cytometry, Confocal imaging

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

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Technical Data

M.Wt:
561.93
Formula:
C27H28N6O.3HCl
Solubility:
Soluble to 50 mM in water and to 50 mM in DMSO
Purity:
≥98%
Storage:
Store at -20°C
CAS No:
875756-97-1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Citations for Hoechst 33342

The citations listed below are publications that use Tocris products. Selected citations for Hoechst 33342 include:

3 Citations: Showing 1 - 3

  1. SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance.
    Authors: Yang Et al.
    Autophagy  2019;:1
  2. Subcutaneous Inoculation of 3D Pancreatic Cancer Spheroids Results in Development of Reproducible Stroma-Rich Tumors.
    Authors: Durymanov Et al.
    Transl Oncol  2019;12:180
  3. Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing.
    Authors: Tabdanov Et al.
    Cell Rep  2018;25:328

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Reviews for Hoechst 33342

Average Rating: 5 (Based on 3 Reviews)

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Hoechst for nuclear staining
By Anonymous on 10/21/2021
Species: Human

Hoechst was used for nuclear staining. Add stain at 1 ug/mL and incubate cells at room temperature for 10 minutes.

Hoechst for nuclear staining 5117
staining nucleus of live cells
By Anonymous on 09/10/2020
Species: Human

I used this dye to stain the nucleus of live cells. After making the stock solution, diluted the dye 1:1000 ratio in the culture media/PBS and added to cells and incubated for 5 mins.

staining nucleus of live cells 5117
Staining nuclei
By Anonymous on 10/27/2019
Species: Human

Add Hoechst dyes at 1 ug/mL.Incubate cells at room temperature for 10 minutes.

Staining nuclei 5117
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