Catalog Number: 5117
Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride
Biological Activity
Cell permeable fluorescent DNA stain; binds minor groove of AT-rich regions. Used to quantify DNA in viable cells.
Technical Data
  • M.Wt:
    561.93
  • Formula:
    C27H28N6O.3HCl
  • Solubility:
    Soluble to 100 mM in water and to 50 mM in DMSO
  • Purity:
    >98%
  • Storage:
    Store at -20°C
  • CAS No:
    23491-52-3
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study.
    Portugal and Waring
    Biochim.Biophys.Acta., 1988;949:158
  2. Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter.
    Loken
    Cytometry, 1980;1:136

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