Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride
Biological ActivityCell permeable fluorescent DNA stain; binds minor groove of AT-rich regions. Used to quantify DNA in viable cells.
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Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study.
Portugal and Waring
Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter.
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Immunostaining of HEK 293 cells