Hoechst Janelia Fluor® 526

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Hoechst Janelia Fluor® 526 | Fluorescent DNA Stains
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Description: Fluorogenic, green-emitting DNA probe. Used for: fixed and live-cell imaging. Application: confocal microscopy, flow cytometry, super resolution microscopy (SRM)
Alternative Names: JF526-HST

Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-((2-(2-(2-(4-(4-(6-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate tetratrifluoroacetate

Purity: ≥95%

Product Details

Biological Activity

Key information: Hoechst Janelia Fluor® 526 is a fluorogenic, green-emitting DNA probe. Suitable for live cell imaging.

Used for: fixed and live-cell imaging.

Application: confocal microscopy, flow cytometry, super resolution microscopy (SRM).

Properties and Photophysical Data: preferentially stains and binds minor groove of AT-rich regions. When bound to A-T DNA, Hoechst Janelia Fluor® 526 exhibits a quantum yield (QY) of 0.126; when bound to G-C DNA Hoechst Janelia Fluor® 526 exhibits a quantum yield of 0.04. Fluorogenic, it fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor® 526 can be combined with fluorogenic red-emitting DNA probe Hoechst Janelia Fluor® 646 (Cat. No. 6804) for multiplexing experiments. Excitation and emission maxima (λ) are 531 nm and 549 nm, respectively; quantum yield = 0.126; extinction coefficient = 118,000 M-1cm-1.

To measure the absorbance spectrum of Hoechst Janelia Fluor® 526 we recommend the following solvent: TFE plus 0.1% TFA.

Scientific Data

Confocal Microscopy Application of Hoechst Janelia Fluor<sup>&#174;</sup> 526 in U2OS cells. View Larger

Application of Hoechst Janelia Fluor® 526 in U2OS cells. Fixed U2OS cells were stained with Hoechst Janelia Fluor® 526 (2 µM) for 1 hour and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 514 nm / emission: 526–615 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus.

Optical Data for Hoechst Janelia Fluor® 526

Hoechst Janelia Fluor<sup>®</sup> 526 Dye Spectra
Emission Color Yellow
λabs 531 nm
λem 549 nm
Extinction Coefficient (ε) 118,000 M-1cm-1
Quantum Yield (φ) 0.126
Application Confocal Microscopy, Flow Cytometry, Super Resolution Microscopy (SRM)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

Soluble to 10 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

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