Hoechst Janelia Fluor® 646

Tocris Bioscience | Catalog # 6804

Fluorogenic, red-emitting DNA probe. Used for: fixed and live-cell imaging. Application: confocal microscopy, super resolution microscopy (SRM) (PALM, PAINT), lattice light sheet microscopy
Tocris Bioscience

Key Product Details

Description

Fluorogenic, red-emitting DNA probe. Used for: fixed and live-cell imaging. Application: confocal microscopy, super resolution microscopy (SRM) (PALM, PAINT), lattice light sheet microscopy

Alternative Names

Hoechst JF 646,JF646-Hoechst

Wavelength

Excitation = 655 nm, Emission = 670 nm
View all Fluorescent DNA Stains »

Product Description

Key information:

Hoechst Janelia Fluor® 646 is a fluorogenic red-emitting DNA probe. Suitable for fixed and live-cell imaging.

Application:confocal microscopy, super resolution microscopy (SRM) (including PALM and PAINT), lattice light sheet microscopy.

Properties and Photophysical Data: Hoechst Janelia Fluor® 646 preferentially stains and binds minor groove of AT-rich regions. The compound can be combined with fluorogenic green-emitting DNA probe Hoechst Janelia Fluor® 526 (Cat. No. 7313) for multiplexing experiments. Hoechst Janelia Fluor® 646 can also be combined with Hoechst Janelia Fluor® 526 to perform dual-color STED using the same depletion laser (λdep = 775 nm). Sub-diffraction spatial resolution STED imaging can be achieved using Hoechst Janelia Fluor® 646 in E. coli cells (~100 nM - 1 μM). Hoechst Janelia Fluor® 646 is a desirable alternative to large oligonucleotide-conjugated antibodies for PAINT experiments, particularly for bacterial studies. Excitation and emission maxima (λ) are 655 nm and 670 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1.

Hoechst Janelia Fluor® 646 is fluorogenic and fluoresces only once bound to DNA, enabling hassle-free no-wash experiments.

To measure the absorbance spectrum of Hoechst Janelia Fluo® 646 we recommend the following solvent: TFE plus 0.1% TFA.

Scientific Data Images for Hoechst Janelia Fluor® 646

Application of Hoechst Janelia Fluor® 646 in U2OS cells.

Application of Hoechst Janelia Fluor® 646 in U2OS cells.

Fixed U2OS cells were stained with Hoechst Janelia Fluor® 646 (1 µM) for 1 hour and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 633 nm / emission: 638–759 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Hoechst Janelia Fluor<sup>®</sup> 646 Dye Spectra
Emission Color Red
Brightness
λabs 655 nm
λem 670 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 640 nm
Cell Permeable Yes
Application Super Resolution Microscopy (PALM, PAINT), Confocal Microscopy, Lattice Light Sheet Microscopy

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Product Specifications for Hoechst Janelia Fluor® 646

Molecular Weight

1119.39

Formula

C64H70N10O7Si

Storage

Store at -20°C

Purity

≥85% (HPLC)

Chemical Name

2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2-(2-(4-(4-(5-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate

CAS Number

2462011-60-3

InChI Key

DTAPBNBSCGEQMS-UHFFFAOYSA-N

SMILES

C[Si]1(C)C2=C/C(C=CC2=C(C3=CC(C(NCCOCCOCCNC(CCCOC4=CC=C(C5=NC6=C(C=C(C=C6)C7=NC8=C(C=CC(N9CCN(C)CC9)=C8)N7)N5)C=C4)=O)=O)=CC=C3C([O-])=O)C%10=CC=C(N%11CCC%11)C=C%101)=[N+]%12CCC/%12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.19 10

Preparing Stock Solutions for Hoechst Janelia Fluor® 646

The following data is based on the product molecular weight 1119.39.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 8.93 mL 44.67 mL 89.33 mL
0.5 mM 1.79 mL 8.93 mL 17.87 mL
1 mM 0.89 mL 4.47 mL 8.93 mL
5 mM 0.18 mL 0.89 mL 1.79 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Hoechst Janelia Fluor® 646

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Hoechst Janelia Fluor® 646

For research use only

Citations for Hoechst Janelia Fluor® 646

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