Hoechst Janelia Fluor® 646

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Hoechst Janelia Fluor® 646 | CAS No. 2462011-60-3 | Fluorescent DNA Stains
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Description: Fluorogenic, red-emitting DNA probe
Alternative Names: Hoechst JF 646, JF646-Hoechst

Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2-(2-(4-(4-(5-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate

Purity: ≥90%

Product Details

Biological Activity

Hoechst Janelia Fluor® 646 is a fluorogenic red-emitting DNA probe; it preferentially stains and binds minor groove of AT-rich regions. The compound can be combined with fluorogenic green-emitting DNA probe Hoechst Janelia Fluor® 526 for multiplexing experiments. Hoechst Janelia Fluor® 646 can also be combined with Hoechst Janelia Fluor® 526 to perform dual-color STED using the same depletion laser (λdep = 775 nm). Sub-diffraction spatial resolution STED imaging can be achieved using Hoechst Janelia Fluor® 646 in E. coli cells (~100 nM - 1 μM).

Hoechst Janelia Fluor® 646 is a desirable alternative to large oligonucleotide-conjugated antibodies for PAINT experiments, particularly for bacterial studies. Hoechst Janelia Fluor® 646 exhibits a quantum yield (QY) of 0.54; extinction coefficicent 152, 000; excitation maximum = 655 nm; emission maximum = 670 nm.

Fluorogenic: fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor® 646 is suitable for multicolor microscopy experiments and for use in live cell imaging.

To measure the absorbance spectrum of Hoechst Janelia Fluo® 646 we recommend the following solvent: TFE plus 0.1% TFA.

Optical Data for Hoechst Janelia Fluor® 646

Hoechst Janelia Fluor<sup>®</sup> 646 Dye Spectra
Emission Color Red
λabs 655 nm
λem 670 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 640 nm
Cell Permeable Yes
Application Super Resolution Microscopy (PALM, PAINT), Confocal microscopy, Lattice Light Sheet microscopy

Technical Data

Soluble to 10 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Background References

  1. A general method to fine-tune fluorophores for live-cell and in vivo imaging.
    Grimm et al.
    Nat.Methods, 2017;14:987

Product Datasheets

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