HPRT Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-29542

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HPRT Primary Antibodies »

Product Specifications

Immunogen

Between 168 and 218

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HPRT Antibody - BSA Free

HPRT Antibody

Immunoprecipitation:HPRT AntibodyNBP3-29542] -

Immunoprecipitation:HPRT AntibodyNBP3-29542] -Detection of human HPRT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HPRT1 antibody NBP3-29542 (lot NBP3-29542-1) used for IP at 6 µg per reaction. HPRT1 was also immunoprecipitated by rabbit anti-HPRT1.. For blotting immunoprecipitated HPRT1, NBP3-29542 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for HPRT Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HPRT

HPRT is encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Long Name

Hypoxanthine-Guanine Phosphoribosyltransferase

Alternate Names

HGPRT, HGPRT1, HPRT1

Gene Symbol

HPRT1

Additional HPRT Products

Product Documents for HPRT Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HPRT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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