Recombinant Human HR6B/UBE2B His (N-Term) Protein

Name: Recombinant Human HR6B/UBE2B His (N-Term) Protein
Brand: Novus Biologicals
SKU: NBP2-35088
Price: 2252 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-35088

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Tag

His (N-Term)

Applications

SDS-PAGE

View all HR6B/UBE2B Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to HR6B/UBE2B and 14 amion acid vector sequence including 6x His tag at N-terminusSource: E. coli

Amino Acid Sequence: MHHHHHHAMG QLRSMSTPAR RRLMRDFKRL QEDPPVGVSG APSENNIMQW NAVIFGPEGT PFEDGTFKLV IEFSEEYPNK PPTVRFLSKM FHPNVYADGS ICLDILQNRW SPTYDVSSIL TSIQSLLDEP NPNSPANSQA AQLYQENKRE YEKRVSAIVE QSWNDS

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of HR6B/UBE2B as determined by LAL method.

Predicted Molecular Mass

19.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human HR6B/UBE2B His (N-Term) Protein

SDS-PAGE: Recombinant Human HR6B/UBE2B His (N-Term) Protein [NBP2-35088]

SDS-Page: Human HR6B/UBE2B His (N-Term) Protein [NBP2-35088]

Formulation, Preparation, and Storage

NBP2-35088

Formulation	A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.6 with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
Preservative	5% Trehalose
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Reconstitution	Please contact technical support for detailed reconstitution instructions.
Format	Carrier-Free
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: HR6B/UBE2B

This E2 enzyme encodes for the human homolog of the yeast DNA repair gene RAD6, which is induced by DNA damaging agents. UBE2B can conjugate ubiquitin to histone H2A in an E3- independent manner in vitro, and is essential for the multiubiquitination and degradation of N-end rule substrates. Additionally, UBE2B may have a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia.

Long Name

Ubiquitin-conjugating Enzyme E2B

Alternate Names

E2-17k, UBE2B

Gene Symbol

UBE2B

Additional HR6B/UBE2B Products

Product Documents for Recombinant Human HR6B/UBE2B His (N-Term) Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human HR6B/UBE2B His (N-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

p53 Pathway

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