HRAS Antibody (OTI1D9)

Novus Biologicals | Catalog # NBP2-45745

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D9
View all HRAS Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HRAS(NP_005334) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HRAS Antibody (OTI1D9)

Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]

Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]

Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745]

Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745]

Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]

Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]

Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY vH RAS.
Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]

Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]

Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (10ug) from 8 Human tissue by using HRAS monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745]

Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745]

Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745]

Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745]

Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescence: HRAS Antibody (OTI1D9) [NBP2-45745]

Immunofluorescence: HRAS Antibody (OTI1D9) [NBP2-45745]

Immunofluorescence: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HeLa cells.
HRAS Antibody (OTI1D9)

HRAS Antibody (OTI1D9)[NBP2-45745]

HRAS Antibody (OTI1D9)[NBP2-45745]Simple Western� analysis of endogenous protein MCAM from HELA lysates (0.1 mg/mL) using CD146 (MCAM) Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for HRAS Antibody (OTI1D9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HRAS

Alternate Names

C-BAS/HAS;C-HA-RAS1;C-H-RAS;CTLO;GTPase HRas;HAMSV;HRAS1;H-RASIDX;p21ras;RASH1

Gene Symbol

HRAS

Additional HRAS Products

Product Documents for HRAS Antibody (OTI1D9)

Certificate of Analysis

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Product Specific Notices for HRAS Antibody (OTI1D9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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