HSP27 [p Ser78] Antibody

Novus Biologicals | Catalog # NBP2-24458

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Key Product Details

Species Reactivity

Validated:

Human, Canine, Equine, Primate

Predicted:

Bovine (100%), Chimpanzee (100%), Mouse (100%), Opossum (100%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

A portion of human HSP27 protein containing a phosphorylated serine residue at position 78 was used as the immunogen.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (88%)

Modification

p Ser78

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP27 [p Ser78] Antibody

Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458]

Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458]

Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for HSP27 [p Ser78] Antibody

Application
Recommended Usage

Western Blot

0.01-0.1 ug/ml

Reviewed Applications

Read 1 review rated 3 using NBP2-24458 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

UniProt

P04792

Additional HSP27 Products

Product Documents for HSP27 [p Ser78] Antibody

Certificate of Analysis

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Product Specific Notices for HSP27 [p Ser78] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for HSP27 [p Ser78] Antibody (1)

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  • HSP27 [p Ser78] Antibody
    Name: Byron Baron
    Application: Western Blot
    Sample Tested: MCF7 (breast)
    Species: Human
    Verified Customer | Posted 12/26/2016
    Marker, MCF7 (breast), 231 (breast), Colo320 (colon), HeLa (cervix) (15ug protein per well). Bands only observed for MCF7 and 231.
    Primary rabbit anti HSP27p78 antibody diluted 1:1000 in TBS. Membrane incubation for 2 hours at RT. Secondary IRDye 800CW goat anti mouse antibody diluted 1:10000 in TBS. Membrane incubation for 1 hour at RT. Detection by Li-Cor Odyssey
    HSP27 [p Ser78] Antibody NBP2-24458

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