Rat Hsp47 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-76581

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat HSP47 in samples. No significant cross-reactivity or interference between Rat HSP47 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.04%

Inter-Assay Precision (Precision between assays) CV% 5.18%

Recovery for Rat Hsp47 ELISA Kit (Colorimetric)

Recovery

90-108%

Linearity

Rat Hsp47 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Hsp47 ELISA Kit (Colorimetric)

Rat Hsp47 ELISA Kit (Colorimetric)

ELISA: Rat Hsp47 ELISA Kit (Colorimetric) [NBP2-76581] -

ELISA: Rat Hsp47 ELISA Kit (Colorimetric) [NBP2-76581] - Standard Curve Reference

Kit Contents for Rat Hsp47 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HSP47

Hsp47 encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis.

Long Name

Heat Shock Protein 47

Alternate Names

AsTP3, BERF-1, CBP1, CBP2, Colligin, gp46, OI10, PPROM, RA-A47, Serpin H1, SERPINH1

Gene Symbol

SERPINH1

Additional HSP47 Products

Product Documents for Rat Hsp47 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Rat Hsp47 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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